Project Number: 5348-21000-024-03
Project Type: Specific Cooperative Agreement

Start Date: Aug 25, 2005
End Date: Jun 30, 2008

Objective:
Screen the lentil collection at Corvallis, Oregon for Pea enation mosaic virus where consistent disease pressure is available yearly. Screen lentil and pea collections for Sclerotinia sclerotiorum.

Approach:
Five hundred accessions from the P.I. collection of lentil germplasm will be planted. We will rely on natural infection vectored by aphids to inoculate the plots. The accessions will be scored for symptoms of PEMV infection using a 1 to 5 scale. Samples of diseased plants will be assayed using ELISA to verify the presence of pea enation mosaic virus. The field trial will include germplasm accessions previously scored as being resistant in field and greenhouse screening tests. Other viruses present in the plot area will also be determined through 'trap' plantings of faba bean. Data on reactions of the accessions to the virus will be reported and entered into the GRIN system. Documents SCA with Oregon State University. Formerly 5348-21000-014-07S (5/08).