Project Number: 5366-21000-027-05
Project Type: Specific Cooperative Agreement

Start Date: Aug 05, 2003
End Date: Aug 04, 2008

Objective:
Evaluate the reaction of NSGC barley accessions to established races of barley stripe rust (Puccinia striiformis f.sp. hordei) endemic in the Central Valley of California.

Approach:
NSGC winter-habit barley accessions and accessions which have exhibited resistance to barley stripe rust in previous tests in Bolivia will be selected for evaluation in the first years of the project. Spring-habit accessions will be evaluated in the succeeding years of the project. UC Davis researchers will plant the accessions in the Davis, California area or other Central Valley locations where a natural epidemic of barley stripe rust can be expected. Data collected will be submitted to ARS for entry into GRIN. Documents SCA with UC Davis. Fine mapping of corn or rice MIPS and low phytic acid 1 loci will use large F2 populations (500 to >1000 individuals) to identify markers closely linked to a given locus. Smaller recombinant inbred line populations will also be used to map new loci and to check map positions of selected clones. Bacterial Artificial Chromosome (BAC) libraries will be screened for those containing selected MIPS or low phytic acid 1 loci. Selected BACs will be sequenced in part (sequencing of selected subclones) or in whole. Corn expression libraries, prepared from selected tissues, will be screened for clones encoding specific kinases. Kinase clones will be mapped using above methods. Formerly 5366-21000-020-06S (6/03) 5366-21000-022-04S (5/08).