2007 Annual Report 1a.Objectives (from AD-416) Evaluate relationship between mapped genetic markers and cytological location on carrot chromosomes. 1b.Approach (from AD-416) 1) Data and locating at least 10 specific genetic (pigment, nematode resistance) markers on carrot chromosomes. 2) Data locating 3 general chromosome markers. 3) Preliminary evaluation of chromosome marker variation in genetic stocks and diverse germplasm of potato and cucurbit. 3.Progress Report This report serves to document accomplishments under a specific cooperative agreement between ARS and The University of Wisconsin. Additional details of research can be found in the parent project 3655-21000-037-00D. Fluorescent in situ hybridization was successful in characterizing ten Daucus species, including carrot, and nine other Apiaceae for location of both nucleolar organizer region and the 5S ribosomal element. Broad diversity was observed. Research in localizing carrots chromosome regions related to bacterial artifical chromosomes is progressing to make progress in characterizing the relationship between the carrot genetic map and chromosomes. Project monitored by meetings and e-mail discussions of progress.