2007 Annual Report
1a.Objectives (from AD-416)
1) Evaluate cold tolerance of winter wheat recombinant inbred and near isogenic wheat populations by quantifying cold tolerance per se, and vernalization response.. 2)Identify QTL's associated with those traits and develop molecular markers for use in marker-assisted selection for cold tolerance.. 3)Fine map the frost resistance gene (Fr2) on chromosome 5A in diploid Triticum monococcum.. 4)Characterize the CBF gene family indiploid wheat, determine which CBF genes are linked to Fr2, and compare locations of CBF genes with locations of QTL's for cold tolerance identified above.
1b.Approach (from AD-416)
Methods used include artificial freeze tests, determination of flowering response under increasing daylight regines, linkage analysis using microsatellites, AFLPs, RFLPs, SNPs and candidate gene sequeces, analysis of diploid wheat BAC libraries. Statistical methods used include analysis of variance and maximum likelihood estimation of linkage. Results will provide genetic resources for mapping and cloning cold tolerance genes in wheat and more efficient methods of selection for cold tolerance. Results will also be applicable to other crops (for example winter barley). Documents Reimbursable with NRI. Log 25968.
3.Progress Report
This report serves to document research conducted under a reimbursable agreement between USDA-CSREES-NRI and the USDA-ARS. Additional details of research can be found in the report for parent CRIS PROJECT NUMBER: 5348-21220-003-00D, IDENTIFICATION AND DEPLOYMENT OF GENES TO REDUCE PRODUCTION RISKS AND IMPROVE QUALITY IN CLUB AND SOFT WHITE WINTER WHEAT. Quantitative trait loci (QTLs) for cold tolerance and vernalization requirement have been identified in two winter wheat populations, Norstar/Centurk78 and Karl/Z0031 on chromosome 5A that overlap the region containing CBF gene homologues that are associated with cold tolerance in other species. The problem being addressed is genetic control of winter survival in winter wheat. This project is being accomplished by Dr. Latha Reddy who is working in Dr. Garland Campbellās laboratory. Results of this research will improve efforts to reduce grower risk of winter injury in wheat and the QTLs will be used to select for more cold tolerant winter wheat. Progress on the collaborative project is monitored through monthly meetings with other scientists and through emails containing data and photographs.
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