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![](https://webarchive.library.unt.edu/eot2008/20081108202730im_/http://www.ars.usda.gov/incme/images/Research_head.gif) |
Research Project:
SOYBEAN BAC-CLONE IDENTIFICATION USING OVERGO HYBRIDIZATION
Location: Soybean Genomics and Improvement
2006 Annual Report
4d.Progress report.
This report serves to document research conducted under a specific cooperative agreement between ARS and the Department of Agronomy, Purdue University and is a subcontract derived from a grant from the United Soybean Board (#4239) and supports the objectives of parent project 1275-21000-164-00D. Additional details of the research can be found in the report for the parent project 1275-21000-164-00D (A Single Nucleotide Polymorphism-based Map of Soybean and Applications to Gene Discovery in Germplasm). The objectives of this agreement are to develop and employ an overgo (OVERlapping oliGOnucleotides) hybridization system for the identification of soybean bacterial artificial chromosome (BAC) clones that contain specific DNA sequences. The DNA sequences to be used in the design of the overgo hybridization probes will be derived from DNA markers that have been positioned on the genetic map of soybean or that will be positioned in the near future. More than 1000 overgo hybridizations to two soybean BAC libraries were successfully completed. The BAC clones identified by the overgo probes will be important in anchoring the soybean physical map to the genetic map. The information relating to the positions of the BAC-clones identified by the overgo probes is being incorporated into a web-based analysis system called CMap which is a comparative genetic mapping tool that is part of the Generic Software Components for Model Organism Databases (GMOD). With CMap it will be possible to view the position of BAC clones and BAC-contigs on the genetic map of the soybean genome.
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Last Modified: 11/07/2008
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