2004 Annual Report 4.What were the most significant accomplishments this past year? This report serves to document research conducted under a Specific Cooperative Agreement between ARS and Texas A&M University. Additional details of research can be found in the report for the parent CRIS 6406-21000-009-00D, Enhancing Corn with Resistance to Aflatoxin Contamination and Insect Damage. This is a new project that was initiated in May 2004. Research has been initiated to assess the effects of 9-LOX gene mutants on resistance to aflatoxin contamination in corn. Development of near-isogenic 9-LOX mutant and wild type corn lines needed for these studies has begun. Molecular analysis will be used to investigate expression of Mu-insertions in the LOX genes and their effects on aflatoxin contamination.