2003 Annual Report 4.What were the most significant accomplishments this past year? This report serves to document rersearch conducted under a trust received by USDA-ARS from the North American Millers Assoication. Additional details can be found in the report for the parent project 5348-21000-016-00D. In order to determine genetic relationships amoung scab resistant germplasm accessions, we are screening molecular markers on 20 parents with varying degress of resistance to scab. We are using both resistance gene analog polymorphisms and microsatelite markers for this analysis, have detected several polymorphic differences among the the accessions, and we will determine genetic relatedness among those lines as well as determine the association between markers and resistance to scab. We are also assessing agronomic traits in a population with Wang shu bai as the scab resistance donor in collaboration with Sharyar Kianian at North Dakota State University. Our goal is to develop molecular markers useful in breeding for resistance to scab as well as to develop markers and sscab resistant germplasm with superior agronomic and end use quality.