Project Number: 3635-31320-008-10
Project Type: Reimbursable

Start Date: Oct 01, 2006
End Date: Aug 31, 2008

Objective:
The focus of this project is to understand the role of the chicken genes that confer either genetic resistance and/or vaccinal immunity. Specifically, we propose to develop genomics tools and utilize multiple approaches to identify genes and pathways for genetic resistance to Marek's disease (MD), or vaccinal response to MD vaccines. In related experiments, significant efforts are designed to gain knowledge on the role of the MHC in viral antigen presentation for resistance or vaccinal immunity. Furthermore, a method to produce a chicken line optimized for embryo derived vaccines or transgenic chicken production will be tested. Success of this project will generate molecular markers and knowledge that will hasten the development of highly disease resistant commercial chickens, and the ability to produce safe vaccines with high efficacy. This project will also be the focal point for coordinated efforts with the ADOL Marek's Disease CRIS Project and several leading university groups working on poultry genomics, poultry diseases and disease resistance.

Approach:
Genetic markers and ESTs included on DNA microarrays will be placed on the physical map to support contig development and map integration. Individual genes that contribute to genetic resistance to Marek's disease or are involved in pathways of disease resistance will be identified by a combination of whole-genome QTL scans, fine mapping in recombinant congenic strains, DNA microarrays, and viral-host protein interactions. MHC-restricted cytotoxic T lymphocyte and FACS assays will be used to determine the peptide epitopes relevant for antigen presentation and the response of the immune system to viral challenge. Other immunological assays will help characterize mechanisms of immune evasion. Finally, classical breeding assisted by molecular selection will enable the generation of specialized chicken lines. BSL-2; recertified 8/11/06 valid through 8/10/09.