Projects

UNDERSTANDING SOIL-PLANT-HUMAN/ANIMAL FOOD SYSTEMS AND NUTRIENT BIOAVAILABILITY TO IMPROVE HUMAN HEALTH Appropriated (D)   Accession Number: 408012

BIOAVAILABILITY OF FE AND ZINC IN BIOFORTIFIED CROPS Grant (G)   Accession Number: 408158

BIOFORTIFIED CROPS FOR IMPROVED HUMAN NUTRITION Reimbursable (R)   Accession Number: 409487

IMPROVING THE MICRONUTRIENT BIOAVAILABILITY OF STAPLE FOODS, FOOD INGREDIENTS AND FOOD PRODUCTS Specific Cooperative Agreement (S)   Accession Number: 410002

Publications

Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model - (Peer Reviewed Journal) - (08-Jul-08)

Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Ferritin-Iron Bioavailability as Determined Using an In Vitro Digestion/Caco-2 Cell Model - (Peer Reviewed Journal) - (10-Jun-08)

Characterization of Caco-2 and HT29-MTX Co-cultures in an In Vitro Digestion/Cell Culture Model used to Predict Iron Bioavailability - (Peer Reviewed Journal) - (01-Jun-08)

Iron bioavailability to piglets from red and white common beans (Phaseolus vulgaris L.) - (Peer Reviewed Journal)

Tan, S., Yeung, C., Glahn, R.P., Welch, R.M., Lei, X., Miller, D.D. 2008. Iron bioavailability to piglets from red and white common beans (Phaseolus vulgaris L.). Journal of Agriculture and Food Chemistry.

Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells - (Peer Reviewed Journal)

Laparra, J., Tako, E., Glahn, R.P., Miller, D. 2008. Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells. Journal of Agricultural and Food Chemistry. Volume 56:2846-2851

Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based, probiotic-containing, yogurts but incubation at 37 oC does - (Peer Reviewed Journal)

Laparra, J., Tako, E., Glahn, R.P., Miller, D. 2008. Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based, probiotic-containing, yogurts but incubation at 37 oC does. Food Chemistry. Volume 109:122-128.

Genetic and biochemical analysis of iron bioavailability in maize - (Abstract)

Hoekenga, O., Mwaniki, A., Buckler Iv, E.S., Glahn, R.P., Kochian, L.V. 2007. Genetic and biochemical analysis of iron bioavailability in maize (abstract). Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting. Session 176:4

Genetic and biochemical analysis of iron bioavailability in maize - (Abstract)

Hoekenga, O., Mwankini, A., Buckler Iv, E.S., Glahn, R.P., Kochian, L.V. 2007. Genetic and biochemical analysis of iron bioavailability in maize. American Society of Agronomy Meetings. Volume 52:102.

Screening of iron bioavailability patterns in eight bean (Phaseolus vulgaris L.) genotypes using the Caco-2 cell in vitro model - (Peer Reviewed Journal)

Ariza-Nieto, M., Blair, M., Welch, R.M., Glahn, R.P. 2007. Screening of iron bioavailability patterns in eight bean (Phaseolus vulgaris L.) genotypes using the Caco-2 cell in vitro model. Journal of Agricultural and Food Chemistry. 55:7950-7956.

Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine - (Peer Reviewed Journal)

Tako, E., Glahn, R.P., Welch, R.M., Lei, X., Yasuda, K., Miller, D. 2008. Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine. British Journal of Nutrition. Volume 99:472-480.

Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions - (Peer Reviewed Journal)

Beisiegel, J.M., Hunt, J.R., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B. 2007. Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions. American Journal of Clinical Nutrition. 86:388-396.

A COMPARISON OF PHYSICAL PROPERTIES, SCREENING PROCEDURES AND A HUMAN EFFICACY TRIAL FOR PREDICTING THE BIOAVAILABILITY OF COMMERCIAL ELEMENTAL IRON POWDERS USED FOR FOOD FORTIFICATION - (Peer Reviewed Journal)

Lynch, S.R., Bothwell, T., Campbell, L., Cowan, K., Glahn, R.P., Hallberg, L., Hoppe, M., Hulthen, L., Hunt, J.R., Hurrell, R.F., Miller, D., Swain, J., Turner, L., Winichagoon, P., Yeung, C.K., Zeder, C., Zimmermann, M.B. 2007. A comparison of physical properties, screening procedures and a human efficacy trial for predicting the bioavailability of commercial elemental iron powders used for food fortification. International Journal for Vitamin and Nutrition Research. 77:107-124.

Kaempferol in red and pinto bean seed (PHASEOLUS VULGARIS L.) coats inhibits iron bioavailability using an in vitro digestion/human CACO-2 cell model - (Peer Reviewed Journal)

Hu, Y., Cheng, Z., Heller, L., Krasnoff, S.B., Glahn, R.P., Welch, R.M. 2006. Kaempferol in red and pinto bean seed (PHASEOLUS VULGARIS L.) coats inhibits iron bioavailability using an in vitro digestion/human CACO-2 cell model. Journal of Agricultural and Food Chemistry. 54:9254-9261.

Moving toward a more physiological model: application of mucin to refine the in vitro digestion/Caco-2 cell culture system - (Peer Reviewed Journal)

Jin, F., Welch, R.M., Glahn, R.P. 2006. Moving toward a more physiological model: application of mucin to refine the in vitro digestion/Caco-2 cell culture system. Journal of Agricultural and Food Chemistry. 54:8962-8967.

Iron bioavailability from maize and beans: a comparison of human and Caco-2 cell measurements - (Abstract)

Beisiegel, J.M., Hunt, J.R., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B. 2007. Iron bioavailability from maize and beans: a comparison of human and Caco-2 cell measurements [abstract]. Program Abstracts, Consequences and Control of Micronutrient Deficiencies: Science, Policy, and Programs - Defining the Issues. April 16-18, 2007. Istanbul, Turkey. p. 103.

BREAD BAKING DOES NOT ALTER THE BIOAVAILABILITY OF HYDROGEN-REDUCED IRON POWDER ADDED TO REFINED WHEAT FLOUR - (Peer Reviewed Journal)

Maekawa, A.A., Glahn, R.P., Lei, X., Miller, D. 2006. Bread baking does not alter the bioavailability of hydrogen-reduced iron powder added to refined wheat flour. Journal of Agricultural and Food Chemistry. 54:8362-8368.

IRON DISSOCIATES FROM THE NAFEEDTA COMPLEX PRIOR TO OR DURING INTESTINAL ABSORPTION IN RATS - (Peer Reviewed Journal)

Zhu, L., Yeung, C., Glahn, R.P., Miller, D. 2006. Iron dissociates from the nafeedta complex prior to or during intestinal absorption in rats. Journal of Agricultural and Food Chemistry. 54:7929-7934.

IRON UPTAKE BY CACO-2 CELLS FROM NAFEEDTA AND FESO4: EFFECTS OF ASCORBIC ACID, PH, AND A FE(II) CHELATING AGENT - (Review Article)

Zhu, L., Yeung, C., Miller, D., Glahn, R.P. 2006. Iron uptake by caco-2 cells from nafeedta and feso4: effects of ascorbic acid, ph, and a fe(ii) chelating agent. Journal of Agricultural and Food Chemistry. 54:7924-7928.

IRON (FE) BIOAVAILABILITY AND THE DISTRIBUTION OF ANTI-FE NUTRITION BIOCHEMICALS IN THE UNPOLISHED, POLISHED GRAIN AND BRAN FRACTION OF FIVE RICE GENOTYPES - (Peer Reviewed Journal)

Prom-U-Thai, C., Glahn, R.P., Welch, R.M., Huang, L., Rerkasem, B. 2006. Iron (fe) bioavailability and the distribution of anti-fe nutrition biochemicals in the unpolished, polished grain and bran fraction of five rice genotypes. Journal of the Science of Food and Agriculture. 86(8):1209-1215.

A CACO-2 CELL MODEL PREDICTS RELATIVE IRON ABSORPTION FROM TROPICAL MAIZE BY WOMEN - (Abstract)

Beisiegel, J.M., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B., Hunt, J.R. 2006. A caco-2 cell model predicts relative iron absorption from tropical maize by women [abstract]. FASEB J. 20(4):A624.

MEAT AND ASCORBIC ACID CAN PROMOTE FE AVAILABILITY FROM FE-PHYTATE BUT NOT FROM FE-TANNIC ACID COMPLEXES - (Peer Reviewed Journal)

Engle-Stone, R., Yeung, A., Welch, R.M., Glahn, R.P. 2005. Meat and ascorbic acid can promote fe availability from fe-phytate but not from fe-tannic acid complexes. Journal of Agricultural and Food Chemistry. 53:10276-10284.

ENDOSPERM SPECIFIC CO-EXPRESSION OF RECOMBINANT SOYBEAN FERRITIN AND ASPERGILLUS PHYTASE IN MAIZE RESULTS IN SIGNIFICANT INCREASES IN THE LEVELS OF BIOAVAILABLE IRON - (Peer Reviewed Journal)

Drakakaki, G., Marcel, S., Glahn, R.P., Lund, L., Periagh, S., Christou, P., Stoger, E. 2005. Endosperm specific co-expression of recombinant soybean ferritin and aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Molecular Biology. 59(6):869-880.

THE USEFULNESS OF INVITRO MODELS TO PREDICT THE BIOAVAILABILITY OF IRON AND ZINC: A CONSENSUS STATEMENT FROM THE HARVESTPLUS EXPERT CONSULTATION - (Other)

Fairweather-Tait, S., Lynch, Sean, Hunt, J.R., et al. 2005. The usefulness of in vitro models to predict the bioavailability of iron and zinc: a consensus statement from the HarvestPlus expert consultation. International Journal for Vitamin and Nutrition Research.75(6):371-374.

PREBIOTICS AND IRON BIOAVAILABILITY - IS THERE A CONNECTION?¿ - (Literature)

Yeung, C., Miller, D., Welch, R.M., Glahn, R.P. 2005. Prebiotics and iron bioavailability - is there a connection?¿. Journal of Food Science. 70:1288-1292.

BIOAVAILABILITY OF ELEMENTAL IRON POWDERS IN BREAD ASSESSED WITH AN IN VITRO DIGESTION/CACO-2 CELL CULTURE MODEL - (Peer Reviewed Journal)

Yeung, C., Miller, D.D., Zhiqiang, C., Glahn, R.P. 2005. Bioavailability of elemental iron powders in bread assessed with an in vitro digestion/caco-2 cell culture model. Journal of Food Science. 70:5199-5203.

GENOTYPE-ENVIRONMENT INTERACTIONS AND STABILITY ANALYSES OF KERNEL-IRON AND -ZINC CONCENTRATIONS IN TROPICAL MAIZE - (Peer Reviewed Journal) - (04-Nov-04)

INHIBITION OF IRON UPTAKE FROM IRON SALTS AND CHELATES BY DIVALENT METAL CATIONS IN INTESTINAL EPITHELIAL CELLS - (Peer Reviewed Journal)

Yeung, C.K., Glahn, R.P., Miller, D. 2004. Inhibition of iron uptake from iron salts and chelates by divalent metal cations in intestinal epithelial cells. Journal of Agricultural and Food Chemistry. Available: http://pubs.acs.org/journals/jafcau/.

IRON AVAILABILITY OF A FORTIFIED PROCESSED WHEAT CEREAL: A COMPARISON OF 14 FE FORMS USING AN IN VITRO DIGESTION/CACO-2 MODEL - (Peer Reviewed Journal)

Wortley, G., Leusner, S., Good, C., Gugger, E., Glahn, R.P. 2004. Iron availability of a fortified processed wheat cereal: a comparison of 14 fe forms using an in vitro digestion/caco-2 model. British Journal of Nutrition. 93:65-71.

ENHANCING THE ABSORPTION OF FORTIFICATION IRON: A SUSTAIN TASK FORCE REPORT - (Other)

Yeung, C., Miller, D., Hurrell, R.F., Lynch, S., Bothwell, T., Cori, H., Hertrampf, E., Kratky, Z., Rodenstein, M., Glahn, R.P. 2004. Enhancing the absorption of fortification iron: a sustain task force report. International Journal for Vitamin and Nutrition Research. 74:387-401.

THE EFFECT OF CALCIUM SALTS, ASCORBIC ACID AND PEPTIC PH ON CALCIUM, ZINC AND IRON BIOAVAILABILITIES FROM FORTIFIED HUMAN MILK - (Peer Reviewed Journal)

Etcheverry, P., Wallingford, J., Miller, D., Glahn, R.P. 2005. The effect of calcium salts, ascorbic acid and peptic ph on calcium, zinc and iron bioavailabilities from fortified human milk. International Journal for Vitamin and Nutrition Research. 75:171-178.

IN VITRO DIGESTION/CACO-2 CELL MODEL FOR IRON BIOAVAILABILITY SHOWS STRONG CORRELLATION WITH HUMAN TRIALS - (Peer Reviewed Journal)

Yun, S., Habicht, J., Miller, D.D., Glahn, R.P. 2004. In vitro digestion/caco-2 cell model for iron bioavailability shows strong correllation with human trials. Journal of Nutrition. 134:2717-2721.

IRON ABSORPTION FROM NAFEEDTA IS DOWN-REGULATED IN IRON-LOADED RATS - (Peer Reviewed Journal)

Yeung, C.K., Zhu, L., Glahn, R.P., Miller, D. 2004. Iron absorption from nafeedta is down-regulated in iron-loaded rats. Journal of Nutrition. 134:2270-2274.

CALCIUM, ZINC AND IRON BIOAVAILABILITIES FROM A COMMERCIAL HUMAN MILK FORTIFIER: A COMPARISON STUDY - (Peer Reviewed Journal)

Etcheverry, P., Wallingford, J.C., Miller, D.D., Glahn, R.P. 2004. Calcium, zinc and iron bioavailabilities from a commercial human milk fortifier: a comparison study. Journal of Dairy Science. 87:3629-3637.

CACO-2 CELL FE UPTAKE FROM HUMAN MILK AND INFANT FORMULA - (Peer Reviewed Journal)

Etcheverry, P., Wissler, J., Wortley, G., Glahn, R.P. 2004. Caco-2 cell fe uptake from human milk and infant formula. Nutrition Research. 24(7):573-579.

CARBOHYDRATE FRACTIONS FROM COOKED FISH PROMOTE IRON UPTAKE BY CACO-2 CELLS - (Peer Reviewed Journal)

Glahn, R.P., Huh, E., Hotchkiss, A.T., Brouillette, J.N. 2004. Carbohydrate fractions from cooked fish promote iron uptake by caco-2 cells. Journal of Nutrition. 134:1681-1689.

ASSESSMENT OF CAROTENOID BIOAVAILABILITY OF WHOLE FOODS USING A CACO-2 CELL CULTURE MODEL COUPLED WITH AN IN VITRO DIGESTION - (Peer Reviewed Journal)

Liu, C., Glahn, R.P., Liu, R.H. 2004. Assessment of carotenoid bioavailability of whole foods using a caco-2 cell culture model coupled with an in vitro digestion. Journal of Agricultural and Food Chemistry. 52:4330-4337.

ASSESSMENT OF IRON BIOAVAILABILITY FROM 20 ELITE LATE-MATURING TROPICAL MAIZE VARIETIES USING AN IN VITRO DIGESTION/CACO-2 CELL MODEL - (Peer Reviewed Journal)

Oikeh, S.O., Menkir, A., Maziya-Dixon, B., Welch, R.M., Glahn, R.P. 2004. Assessment of iron bioavailability from 20 elite late-maturing tropical maize varieties using an in vitro digestion/caco-2 cell model. Journal of the Science of Food and Agriculture. 84:1202-1206.

A LOW MOLECULAR WEIGHT FACTOR IN HUMAN MILK WHEY PROMOTES IRON UPTAKE BY CACO-2 CELLS - (Peer Reviewed Journal)

Etcheverry, P., Miller, D.D., Glahn, R.P. 2004. A low molecular weight factor in human milk whey promotes iron uptake by caco-2 cells. Journal of Nutrition. 134(1):93-98.

IRON "BIOFORTIFICATION" OF TROPICAL MAIZE TO REDUCE HUMAN IRON DEFFICIENCY IN WEST AFRICA - (Peer Reviewed Journal)

OIKEH, S.O., MENKIR, A., MAZIYA-DIXON, B., WELCH, R.M., GLAHN, R.P. 2003. IRON "BIOFORTIFICATION" OF TROPICAL MAIZE TO REDUCE HUMAN IRON DEFFICIENCY IN WEST AFRICA. JOURNAL OF PLANT NUTRITION. 26:2307-2319.

ASSESSMENT OF CONCENTRATIONS OF TRACE MINERALS AND BIOAVAILABLE IRON IN GRAINS OF EARLY-MATURING TROPICAL MAIZE VARIETIES - (Peer Reviewed Journal)

OIKEH, S.O., MENKIR, A., MAZIYA-DIXON, B., WELCH, R.M., GLAHN, R.P. ASSESSMENT OF CONCENTRATIONS OF TRACE MINERALS AND BIOAVAILABLE IRON IN GRAINS OF EARLY-MATURING TROPICAL MAIZE VARIETIES. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2003. v. 51. p. 3688-3694.

IRON BIOAVAILABILITY FROM COMMON RAISIN-CONTAINING FOODS ASSESSED WITH AN IN VITRO DIGESTION/CACO-2 CELL CULTURE MODEL: EFFECTS OF RAISINS - (Peer Reviewed Journal)

Yeung, C.K., Glahn, R.P., Miller, D.D. 2003. Iron bioavailability from common raisin-containing foods assessed with an in vitro digestion/caco-2 cell culture model: effects of raisins. Journal of Food Science. 68(5):1866-1870.

SIMULTANEOUS DETERMINATION OF 45CALCIUM AND 65ZINC UPTAKE BY CACO-2 CELLS - (Peer Reviewed Journal)

Etcheverry, P., Wallingford, J.C., Miller, D.D., Glahn, R.P. 2002. Simultaneous determination of 45calcium and 65zinc uptake by caco-2 cells. Journal of Agricultural and Food Chemistry. 50:6287-6294.

RED GRAPE JUICE INHIBITS IRON BIOAVAILABILITY: APPLICATION OF AN IN VITRO DIGESTION/CACO-2 CELL MODEL - (Peer Reviewed Journal)

BOATO, F., WORTLEY, G.M., LIU, R., GLAHN, R.P. RED GRAPE JUICE INHIBITS IRON BIOAVAILABILITY: APPLICATION OF AN IN VITRO DIGESTION/CACO-2 CELL MODEL. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2002.

IN VITRO IRON BIOAVAILABILITY AND ANTIOXIDANT ACTIVITY OF RAISINS - (Peer Reviewed Journal)

Yeung, C.K., Glahn, R.P., Wu, X., Liu, R.H., Miller, D.D. 2003. In vitro iron bioavailability and antioxidant activity of raisins. Journal of Food Science. 68(2):701-705.