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![](https://webarchive.library.unt.edu/eot2008/20090513083800im_/http://www.ars.usda.gov/incme/images/Research_head.gif) |
Research Project:
CHARACTERIZATION OF HEALTH-RELATED PHYTOCHEMICALS FROM SOY AND OTHER AGRICULTURAL CROPS
Location: New Crops and Processing Technology Research
Title: B-GROUP SOYBEAN SAPONINS INDUCE MACROAUTOPHAGY AND S-PHASE ACCUMULATION IN HCT-15 CELLS
Authors
![item](https://webarchive.library.unt.edu/eot2008/20090513083800im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Ellington, Allison - UNIV OF ILLINOIS | ![item](https://webarchive.library.unt.edu/eot2008/20090513083800im_/http://www.ars.usda.gov/incme/images/bullet.gif) |
Berhow, Mark
| ![item](https://webarchive.library.unt.edu/eot2008/20090513083800im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Singletary, Keith - UNIV OF ILLINOIS |
Submitted to: Experimental Biology
Publication Type:
Abstract
Publication Acceptance Date: April 21, 2004
Publication Date: April 21, 2004
Citation: Ellington, A.A., Berhow, M.A., Singletary, K. 2004. B-group soybean saponins induce macroautophagy and s-phase accumulation in hct-15 cells [abstract]. Experimental Biology 18(4):A127.
Technical Abstract: Experiments were conducted to determine the effects of a purified B-group soybean saponin extract on cell proliferation, cell cycle distribution and programmed cell death in cultures of human HCT-15 colon adenocarcinoma cells. Treatment of cells with the extract at doses of 25-100ppm significantly reduced viable cell numbers after 24 and 48 hours of exposure. Treatment of cells with 100ppm of saponins for 48 hours also resulted in a significant accumulation of cells in S-phase, as well as a significant 1.8-fold increase in the cyclin dependant kinase inhibitor p27KIP1/CIP1 protein expression. Evaluation of treated cells with transmission electron microscopy (TEM) revealed a 4-fold increase in cell morphologies characteristic of Type II non-apoptotic programmed cell death including numerous autophagic vacuoles. Saponin treated cells exhibited a 1.5-fold decrease in phosphorylation of p70S6K at serine 389 indicating that a reduction in mTor activity is associated with induction of macroautophagy. These results thus suggest that soybean saponins exert colon cancer suppressive effects through S-phase cell cycle arrest, along with induction of macroautophagy. Funded by the Illinois Council for Food and Agricultural Research.
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Last Modified: 05/12/2009
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