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Research Project:
PRODUCTION AND STABILIZATION OF LIVING MICROBIAL AGENTS
Location: Crop Bioprotection Research
Title: Hydrophobic and Electrostatic Cell Surface Properties of Blastospores of the Entomopathogenic Fungus Paecilomyces Fumosoroseus
Authors
Submitted to: Colloids and Surfaces B: Biointerfaces
Publication Type:
Peer Reviewed Journal
Publication Acceptance Date: November 21, 2005
Publication Date: December 30, 2005
Citation: Dunlap, C.A., Biresaw, G., Jackson, M.A. 2005. Hydrophobic and electrostatic cell surface properties of blastospores of the entomopathogenic fungus Paecilomyces fumosoroseus. Colloids and Surfaces B: Biointerfaces. 46(4):261-266.
Interpretive Summary: Paecilomyces fumosoroseus (Pfr) is a natural fungal enemy of many insects which are considered pests. This potential to control insect pests has made Pfr an attractive biocontrol candidate. The current study characterizes the surface properties of blastospores of Pfr. Blastospores of Pfr are negatively charged under neutral conditions and are hydrophilic, having a strong affinity for water. Understanding these surface properties allows us to predicate how Pfr will interact with its insect host and the environment. This knowledge can then be used to guide formulation and application decisions, which are important in developing a successful biocontrol product.
Technical Abstract: The physicochemical surface properties of blastospores of the entomopathogenic fungus Paecilomyces fumosoroseus were examined. Contact angle measurements were performed on microbial lawns of Paecilomyces fumosoroseus blastospores to quantify their cell surface energy components. In addition, suspensions of P. fumosoroseus blastospores were characterized with the microbial adhesion to solvents assay. Zeta potential measurements were used to quantify the surface charge and determine the zero potential of the blastospores. The results show the blastospores are best described as having a basic monopolar surface and classified as hydrophilic. Blastospores are also negatively charged under neutral conditions with an isoelectric point of 3.4.
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Last Modified: 05/13/2009
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