155. Complete Genome Sequencing of Shewanella putrefaciens 

Rebecca A. Clayton, John Heidelberg, Kenneth Nealson, Eric Gaidos, Alexandre I. Tsapin, James Scott, J. Craig Venter, and Claire M. Fraser 
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850 
NASA Jet Propulsion Lab, 4800 Oak Grove Drive, Pasadena, CA 91109 
rclayton@tigr.org 

We are midway through the closure process in the complete genome sequencing of Shewanella putrefaciens. Random sequencing was completed in July, 1998, and closure began in August, 1998. We present preliminary annotation of the genome, with whole genome comparisons with other completed microbial genomes. Shewanella putrefaciens has high 16S rRNA sequence similarity to Escherichia coli, and also to Vibrio cholerae, a genome in the final stages of closure at TIGR. approximately half its open reading frames have high sequence similarity to E. coli. About 15% of the S.putrefaciens genome has high sequence similarity with V. cholerae but not with E. coli K12. Analysis of the assemblies suggests that the completed genome size will be approximately 5 Mb.