Melioidosis: An Emerging Infection in Taiwan?

Po-Ren Hsueh,* Lee-Jene Teng,* Li-Na Lee,*† Cheong-Ren Yu,* Pan-Chyr Yang,* Shen-Wu Ho,*† and Kwen-Tay Luh,*
*National Taiwan University Hospital, Taipei, Taiwan; and † National Taiwan University College of Medicine, Taipei, Taiwan

Melioidosis is an infectious disease of humans and animals caused by Burkholderia pseudomallei (1). This organism is widely distributed in rice field soil and in stagnant water throughout the tropics (1-8). Although a major disease in Southeast Asia and northern Australia, melioidosis occurs sporadically throughout the world (often in patients with a history of residence in these disease-endemic areas) (1). Humans are usually infected by traumatic inoculation of the organism from the soil or, rarely, by inhalation or ingestion (1,7). Clinical manifestations are protean, ranging from benign and localized abscess, to severe, community-acquired pneumonia to fatal septicemia (1,3,4,7). The two biotypes of B. pseudomallei are categorized by their ability to assimilate L-arabinose (9). The arabinose non-assimilators (Ara) are virulent and can be isolated from both clinical specimens and the environment, whereas the arabinose assimilators (Ara+) are usually avirulent and mainly found in the environment (9,10). Relapse, recrudescence, or reinfection may occur in immunocompromised patients under inappropriate antimicrobial treatment or after resolution of primary infection (1,7,11,12).

In Taiwan in 1985, melioidosis was first reported in a previously healthy patient with multilobar pneumonia, which developed secondary to his near drowning in the Philippines (13). Since then, only six other cases have been reported (14-18). In our study, an additional eight patients with melioidosis found from 1996 to 2000 by National Taiwan University Hospital (NTUH) were reported and microbiologic characteristics (including biotyping, cellular fatty acid chromatograms, antimicrobial susceptibilities, and genotyping) of the strains isolated from these patients were evaluated.

Materials and Methods

Patients and Bacterial Isolates

From January 1980 to May 2000, eight patients with melioidosis were treated at NTUH, a tertiary-care referral center with 2,000 beds in northern Taiwan, and seven patients reported to have melioidosis were treated at other hospitals in Taiwan. Data on demographics, underlying diseases, travel history, type of infection, antimicrobial treatment, and outcome of these patients were analyzed. Infections were considered indigenous if patients had no history of residence or travel to China, Australia, or Southeast Asia. A total of 17 isolates of B. pseudomallei from various clinical specimens of the eight patients treated at NTUH were studied. These isolates were identified by conventional biochemical methods and confirmed by the API 20NE identification system (Biomerieux, Basingstoke, UK) (19,20).

Susceptibility and Antibiotypes

The following antimicrobial agents were provided by their manufacturers for use in this study: amoxicillin-clavulanate (SmithKline Beecham, Welwyn Garden City, UK); cefotaxime (Marion Merrell Dow, Cincinnati, OH); ceftazidime (Glaxo, Greenford, UK); amoxicillin, cefepime, aztreonam, and amikacin (Bristol-Meyer Squibb, Princeton, NJ); imipenem (Merck Sharp & Dohme, Rahway, NJ); meropenem (Sumitomo Pharmaceuticals, Osaka, Japan); piperacillin-tazobactam (Wyeth-Ayerst Laboratories, Pearl River, NY); flomoxef (Shionogi, Osaka, Japan); trovafloxacin (Pfizer Inc., New York, NY); and ciprofloxacin and moxifloxacin (Farbenfabriken Bayer GmbH, Leverkrusen, Germany). MICs of these antimicrobial agents were determined by the agar dilution method according to guidelines established by the National Committee for Clinical Laboratory Standards (21). Isolates were grown overnight on trypticase soy agar plates supplemented with 5% sheep blood (BBL Microbiology Systems, Cockeysville, MD) at 37°C. Bacterial inocula were prepared by suspending the freshly grown bacteria in sterile normal saline and adjusted to a 0.5 McFarland standard. Mueller-Hinton agar (BBL Microbiology Systems) was used for susceptibility testing. With a Steers replicator, an organism density of 10⁴ CFU/spot was inoculated onto the appropriate plate with various concentrations of antimicrobial agents. Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were included as control strains. Antibiotypes were considered identical if all MICs tested were identical or within a twofold dilution discrepancy (22).

Cellular Fatty Acid Chromatogram and Biotypes

Biotypes of these isolates were identified according to the reaction profiles obtained by API 20NE. Arabinose utilization was determined by growth on minimal salt agar containing L-arabinose (0.2%) (9). Fatty acid methyl esters (FAMEs) of these isolates were analyzed by gas-liquid chromatography using a Hewlett-Packard 5890A (Hewlett-Packard; Avondale, PA) as described previously (22). The software library used to identify the B. pseudomallei was TSA, version 3.9 (Microbial ID Inc., Newark, DE).

Strain Typing

Extraction of genomic DNA and polymerase chain reaction (PCR) for determining random amplified polymorphic DNA (RAPD) patterns generated by arbitrarily primed PCR of the 17 isolates of B. pseudomallei were performed as previously described (22). Three oligonucleotide primers used were: M13 (5'-TTATGTAAAACGACGGCCAG-3'(Gibco BRL products, Gaithersburg, MD). ERIC1 (5'-GTGAATCCCCAGGAGCTTACAT-3' (GIBCO Bethesda Research Labortories Products), and OPH-03 (5'-AGACGTCCAC-3') (OPERON Technologies, Inc., Alameda, CA). To interpret RAPD patterns, both faint and intense bands were included (22). The entire procedure, from bacterial growth to interpretation of RAPD pattern, was repeated three times for each isolate to confirm results. Patterns were considered identical only if they differed by no more than one band. Isolates were defined as being of the same strain (highly related isolates) if they had identical antibiotypes, biotypes, and RAPD patterns.

Results

Clinical Characteristics of Patients

	Figure 1
	Click to view enlarged image Figure 1. Cases of melioidosis in Taiwan, by year of diagnosis. Number above each bar indicates number of episodes with indigenous infection.

From January 1982 to May 2000, 17 episodes of infection caused by B. pseudomallei were diagnosed in 15 patients in Taiwan (Table 1). All but one episode occurred between 1994 and 2000 (Figure 1), indicating that cases have increased substantially in recent years. In these 15 patients, 13 were male; mean age was 64 years (range, 40 to 76 years). Patient 1 acquired pneumonia secondary to his near drowning in the Philippines. Patient 4 had had a fever during his stay in mainland China. Patient 6 had fever and left upper abdominal pain on his arrival in Taiwan after a 4-day trip in Rangoon, Burma. Patient 8 had septicemic melioidosis 5 years after travel to Thailand. The 13 other episodes (76%) were considered idigenous. Of the 11 patients with indigenous melioidosis, occupation was known for 7 patients (patients 9 to 15); none were rice farmers. Of the 15 infected patients, 9 (60%) had underlying diseases (6 had diabetes mellitus, and 3 had chronic obstructive pulmonary disease), 12 patients (80%) had pneumonia (including 10 with bacteremia and 1 with concomitant peritonitis), 2 (13%) had soft-tissue abscess, and 1 (7%) had mycotic aneurysm. Two patients each had two episodes of infection, separated by 8 and 10 months, respectively. Four patients (27%) died of melioidosis. One patient (no. 15), who had pneumonia caused by an organism initially identified as P. aeruginosa and treated at another hospital with ceftazidime and amikacin for 1 month, died on the third day in our hospital of refractory pneumonia complicated by empyema and acute respiratory distress syndrome. Two sets of blood cultures collected upon admission grew B. pseudomallei. Susceptibility results by the routine disk susceptibility test showed the organism was resistant to ceftazidime and amikacin. MICs of the organism to ceftazidime and amikacin were 32 µg/mL and >256 µg/mL, respectively (Table 2).

Bacterial Isolates and Biotypes

All B. pseudomallei isolates had characteristic colonial morphology on trypticase soy agar supplemented with 5% sheep blood (BBL Becton Dickinson, Microbiology, Cockeysville, MD), positive oxidase reaction, and resistance to colistin and gentamicin. Of the 17 isolates (from eight patients), two biochemical profiles based on the results of identification by the API 20NE system were 1156576 (biotype I; citrate negative) and 1156577 (biotype II; citrate positive). All isolates tested were Ara. The probability of identification of each B. pseudomallei biochemical profiles was 99.9%.

All 17 isolates (from eight patients) studied had similar FAME profiles, and all had five major FAMEs (each presenting >3% of the total): 14:0 (tetradecanoic acid), 3-OH-14:0 (3-hydroxytetradecanoic acid), 16:0 (hexadecanoic acid), 17:0cyc (-cis-9, 10-methylenehexadecanoic acid), 2-OH-16:0 (2-hydroxyhexadecanoic acid), 3-OH-16:0 (3-hydroxyhexadecanoic acid), 18:1w7c (cis-11-octadecenoic acid), and 19:0cyc (-cis-11, 12-methyleneoctadecanoic acid). FAME profiles of these isolates were consistent with the identification of B. pseudomallei.

Antimicrobial Susceptibilities and Antibiotypes

MICs of 14 antimicrobial agents for the 17 isolates of B. pseudomallei were determined (Table 2). When MIC breakpoints for susceptibility and resistance used for non-Enterobacteriaceae were applied to B. pseudomallei (21), all isolates were susceptible to amoxicillin-clavulanate, piperacillin-tazobactam, imipenem, and meropenem. Most isolates were intermediate or resistant to ampicillin, flomoxef, cefepime, aztreonam, amikacin, and ciprofloxacin. Ceftazidime had in vitro activity equal to or greater than that of cefotaxime against B. pseudomallei. Two isolates (both from patient 12) showed high resistance to ceftazidime and cefepime and intermediate resistance to cefotaxime. Five antibiotypes (antibiotypes I to V) were identified in the 17 isolates.

RAPD Patterns and Identification

	Figure 2
	Click to view enlarged image Figure 2. Random amplified polymorphic DNA (RAPD) patterns of 16 isolates of Burkholderia pseudomallei generated by arbitrarily primed polymerase chain reaction with the primers M13 (upper panel) and ERIC1 (lower panel)....

Eight RAPD patterns were identified by use of the three primers (Figure 2). RAPD patterns of multiple isolates from the same patients were identical. The two isolates (recovered 10 months apart) from patient 10 belonged to strain 5; two of the three isolates (recovered 8 months apart) from patient 11 were also identical (strain 6; Table 3). Isolates recovered from different patients had distinct RAPD patterns.

Conclusion

Between January 1982 and 1994, one episode of melioidosis was identified in Taiwan. From 1994 to May 2000, 16 more cases occurred. Whether these figures represent an actual increase in B. pseudomallei infections in Taiwan or better recognition of this organism by microbiology laboratories is difficult to clarify. In NTUH, the first clinical isolate of B. pseudomallei was recognized in 1980 (the medical record and isolate are now unavailable). Since then, improved laboratory procedures and increasing alertness of laboratory staffs permit more accurate identification of this organism. However, no B. pseudomallei isolate was identified in our laboratory from 1981 to 1995. Thus, from our vantage point, the observed increase in cases of melioidosis is indeed an emerging problem of the last 5 years.

Most of these infections were indigenous. All strains, whether imported or indigenous, were genetically distinct. Different patients were infected with different strains, indicating that stread of strains (intercontinental or within this island), as with Penicillium marneffei (an another emerging pathogen in Taiwan), did not occur (23). Our data suggest that Taiwan should be considered a melioidosis-endemic area, in addition to China, Australia, and Southeast Asia.

Melioidosis has been called the great mimicker because of its protean clinical features (1,7). The most common clinical sign is an acute pulmonary infection (as in our study), though its chronic pulmonary form often resembles tuberculosis (3,16). When localized, melioidosis may cause abscess formation in skin, soft tissue, joints, and visceral organs (1). Melioidosis can become a latent infection that later (as much as 26 years after initial exposure) reactivates into a full-blown illness (even with acute septicemia) (1,12). The content of Ara B. pseudomallei in the soil of a geographic area has been documented to correlate directly with the incidence of melioidosis in that area (5,6). In our study, all but four patients had no prior exposure to well-known disease-endemic areas. Therefore, the strains of B. pseudomallei they acquired might have originated in Taiwanese soil. Unlike other reports (24), most (87%) of our patients were male. Also, all but one patient with indigenous infection were >65 years, and none were rice farmers or obviously had heavy exposure to soil. Environmental surveys for the presence of this organism in the soil of Taiwan, especially in rice fields, is ongoing; thus far, B. pseudomallei has been isolated from two soil samples (data not shown). Although Ara+ B. pseudomallei has been reported to cause severe infection (10), all isolates causing melioidosis in our study were Ara. Our findings support previous observations (1,5,6).

B. pseudomallei are frequently intrinsically resistant to many antibiotics, including aminoglycosides and first- or second-generation cephalosporins (25,26). Current recommendations for therapy of severe melioidosis include intravenous ceftazidime or imipenem for 10 days to 4 weeks (25,26), followed by maintenance therapy with oral amoxicillin-clavulanate or a combination of trimethoprim-sulfamethoxazole and doxycycline for 10 to 18 weeks (27-31). Cefotaxime and ceftriaxone are both less active than ceftazidime against B. pseudomallei in vivo and in vitro (28). However, the observation of ceftazidime resistance's emerging during treatment has been previously reported (30). Its occurrence in our patient 15 might be related to the presence of an empyema. In areas in which melioidosis is endemic, empirical regimens that contain cefotaxime or ceftriaxone for the treatment of severe community-acquired pneumonia or septicemia may not be appropriate.

Some investigators suggest that melioidosis is a facultative intracellular infection (25). The failure of beta-lactam antibiotics to penetrate intracellular sites and kill nonmultiplying dormant bacteria may explain the frequent relapses of melioidosis after treatment with these drugs (25). On the other hand, relapse is documented to be less common (10% versus 30%) in patients who complete a full course of antibiotic treatment (32). Our two patients who had recurrent infections both received oral ciprofloxacin for 2 or 8 weeks. Nearly all isolates had MICs ranging from 1 g/mL to 4 g/mL for the three newer fluoroquinolones tested. Further studies are needed to determine the clinical efficacy of these newer fluoroquinolones for treating melioidosis and their role in preventing future relapse.

Several molecular typing methods have been applied to B. pseudomallei to evaluate the strain relationship in isolates recovered from humans and environment (33-37). Among these methods, RAPD typing has also been documented to be useful for analyzing isolates that cause recurrent infection or reinfection (35). In our study, RAPD typing using three primers clearly demonstrated the genetic diversity of isolates from different patients (with either imported or indigenous infections). In addition, this method showed that multiple isolates from the same patient and isolates causing recurrent infections were genetically identical. The microbial identification system, based on cellular FAME analysis by use of gas chromatography, is an established method for identifying species of bacteria and fungi and showing clustering in bacterial and fungal strains (22,23). Although all our isolates of B. pseudomallei had identical FAME profiles, cluster analysis of these esters (data not shown) failed to provide acceptable discriminatory power for typing the isolates.

In conclusion, Taiwan should be included an as endemic area of melioidosis, and physicians managing patients in Taiwan should be alert to the possibility that this organism might cause community-acquired pneumonia and sepsis.

Dr. Hsueh is an assistant professor in the departments of Laboratory Medicine and Internal Medicine at National Taiwan University College of Medicine in Taipei, Taiwan. His research interests include epidemiology of emerging and nosocomial infections and molecular mechanisms of antimicrobial drug resistance. He is actively involved in developing a national research program for antimicrobial drug resistance in Taiwan (Surveillance for Multicenter Antimicrobial Resistance in Taiwan - SMART).

Address for correspondence: Kwen-Tay Luh, Department of Laboratory Medicine, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan; fax: 886-2-23224263; e-mail: luhkt@ha.mc.ntu.edu.tw

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