![[logo] US EPA](https://webarchive.library.unt.edu/eot2008/20081106094030im_/http://www.epa.gov/epafiles/images/logo_epaseal.gif){kind=logo}
WMI-2000
TECHNICAL PRODUCT BULLETIN #B-19
USEPA, OIL PROGRAM CENTER
ORIGINAL LISTING DATE: JUNE 18, 1990
REVISED LISTING DATE: JANUARY 11, 1996
"WMI-2000"
USEPA, OIL PROGRAM CENTER
ORIGINAL LISTING DATE: JUNE 18, 1990
REVISED LISTING DATE: JANUARY 11, 1996
"WMI-2000"
| I. NAME, BRAND, OR TRADEMARK |
|
WMI-2000 Type of Product: Biological Additive |
| II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT |
|
WMI International, Inc. 4901 Milwee, Suite 109 Houston, TX 77092 Phone: (713) 956-4001 Evening: (713) 526-5829 Fax: (713) 956-7305 Email: wmi@wt.net (Mr. Frank Lemmond) |
| III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS |
|
WMI International, Inc. 4901 Milwee, Suite 109 Houston, TX 77092 Phone: (713) 956-4001 Evening: (713) 526-5829 Fax: (713) 956-7305 Email: wmi@wt.net (Mr. Frank Lemmond) |
| IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION |
|
1. Flammability: WMI-2000 is non-flammable. 2. Ventilation: Avoid breathing dust or aerosol due to possibility of sensitization. Use adequate ventilation. 3. Skin and eye contact; protective clothing; treatment in case of contact: Minimize contact with eyes, skin, and clothing. Wash hands thoroughly after handling culture. 4.a. Maximum storage temperature: 100ºF 4.b. Minimum storage temperature: 35ºF 4.c. Optimum storage temperature range: 45ºF - 90ºF Store in a cool, dry location. Keep partially-used containers tightly closed. Prolonged exposure to high temperature and humidity may lower activity of product. 4.d. Temperatures of phase separations and chemical changes: NA |
| V. SHELF LIFE |
| The shelf life of WMI-2000 is 2 years if stored at ambient temperatures |
| VI. RECOMMENDED APPLICATION PROCEDURE |
|
1. Application Method: The culture should be activated for 2 hours in water, when applicable. Conditions for use vary depending on substrate concentration and type, pH, temperature, availability of nutrients, oxygen content, and liquid medium (static or flowing). 2. Concentration/Application Rate: Inoculation concentration is 5-9 billion spores per gram. 3. Conditions for Use: Temperature requirements are between 35ºF and 100ºF. The optimum dissolved oxygen content is 2 ppm at the sludge-to-water interface. Nitrogen and phosphorous are key nutrients which promote cultural growth, and should be maintained at concentrations of 15-20 ppm and 2.5-5 ppm, respectively. The optimum pH range is between 7.0 and 8.0. |
| VII. TOXICITY AND EFFECTIVENESS | ||||||||||||||||||||||||||
|
a. Toxicity: NA b. Effectiveness: Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994: Summary Data Table:
Results of Gravimetric Analysis: Percentage (%) Decrease in Weight of Oil on Day 28 Control: 0% Nutrient: 25% Product (WMI-2000): 44% |
| VIII. MICROBIOLOGICAL ANALYSIS |
|
1. Listing of all microorganisms by species and percentage in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additives: Optimum pH: 7.0 Optimum temperature: 45ºF - 90ºF Optimum salinity: Fresh water to sea water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 6.0 - 8.0 Temperature: 40ºF - 120ºF Salinity range: Fresh to sea water 4. Special nutrient requirements: none 5. Test results regarding the determination of the following: Salmonella - Negative Fecal Coliform - Negative Shigella - Negative Staphylococcus Coagulase positive - Negative Beta hemolytic streptococcus - Negative |
| IX. PHYSICAL PROPERTIES |
| NA |
| X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS |
| NA |