Optical Methods and Energy Transfer Characterization

Contact: Oleg Gang, Mircea Cotlet

A broad range of optical methods is available for characterization of energy transfer phenomena down to single molecular/particle level. Our capabilities include time- and spectrally resolved bulk/single molecule confocal fluorescence microscopy and fluorescence correlation spectroscopy.

Capabilities

• Confocal Laser Scanning Fluorescence Microscope (CLSFM); Leica SP5
  Contact: Mircea Cotlet
  The instrument provides imaging with spectra and time resolved capabilities. It includes multi-positional navigation of fields of interest, live-imaging with; multi-channel detection, all motorized operations; rotating, panning, zooming and spectral imaging. AOBS system allows for continuous software-based tuning of the spectral characteristic of filters. Supported techniques: FRET, FLIM and Fluorescence Correlation spectroscopy. Software supports measurements and data analysis for all techniques listed above. The CLSFM system is equipped with visible (Argon-ion, 457nm, 476nm, 488nm, 496nm and 514nm and He-Ne, 543nm, 632nm) and NIR (Ti:Sapphire MaiTai, 710-990nm) lasers which are used for one- and two-photon excitation of various types of fluorescent specimens. Laser energizing, microscope initialization and data acquisition, all are software controlled.



• Cuvette Lifetime Fluorometer, ISS PC1
  Contact: Dmytro Nykypanchuk
  The Multifrequency Cross-Correlation Phase and Modulation Fluorometer is capable of steady state and life-time measurements. Lifetime measurement capabilities are from 1 millisecond to 1 picosecond. Includes precise polarization measurements, full automation of all instrument components (cuvette holder, polarizers, shutters, filterwheel, monochromators, and stirrers), and sample temperature control.



• Multiparametric Single Molecule Confocal Microscope
  Contact: Mircea Cotlet
  The CFN developed instrument provides capability to measure optical phenomena on single molecule/particle level and support the range of methods, including two-color excitation/two-color detection, polarized single molecule detection, burst integrated fluorescence lifetimes (BIFL), fluorescence correlation spectroscopy (FCS), scanning stage fluorescence imaging, fluorescence lifetime imaging (FLIM), spectrally resolved fluorescence detection.

Last Modified: May 6, 2008
Please forward all questions about this site to: Stephen Giordano.

One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE’s Office of Science by Brookhaven Science Associates, a limited-liability company founded by Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit, applied science and technology organization.

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