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Research Project:
PHYSIOLOGICAL AND GENETIC BASIS OF COTTON ACCLIMATION TO ABIOTIC AND BIOTIC STRESS
Location: Plant Physiology and Genetics Research
Title: AN INTEGRATED SSR-STS-SRAP-RAPD GENETIC MAP USING RECOMBINANT INBRED LINE POPULATION IN TETRAPLOID COTTON
Authors
 | Lu, Yinggzhi - NMSU, LAS CRUCES, NM | | Zhang, Jinfa - NMSU, LAS CRUCES, NM | |
Percy, Richard
| | Cantrell, R - COTTON INC, CARY NC |
Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type:
Proceedings/Symposium
Publication Acceptance Date: January 9, 2004
Publication Date: June 1, 2004
Citation: Lu, Y., Zhang, J., Percy, R.G., Cantrell, R.G. 2004. An integrated ssr-sts-srap-rapd genetic map using recombinant inbred line population in tetraploid cotton. National Cotton Council Beltwide Cotton Conference. 1156-1161.
Interpretive Summary: An upland cotton germplasm, NM24016 derived from an interspecific cross between Gossypium hirsutum and G. barbadense was used to cross with Pima 3-79 to develop a recombinant inbred line (RIL) population. A total of 166 markers, consisting of SSRs, RAPDs, STSs, and SRAPs were developed for the mapping population. Twenty-eight linkage groups were constructed from 142 markers. Comparative marker mapping between F2 and RIL populations indicated a linkage map expansion in the RIL population, which strongly supports the strategy to develop the RIL population for more accurate genetic mapping. Other 24 markers that are polymorphic between the two parents did not segregate in the RIL population, indicating selective elimination on the related chromosomal regions.
Technical Abstract: An upland cotton germplasm, NM24016 derived from an interspecific cross between Gossypium hirsutum and G. barbadense was used to cross with Pima 3-79 to develop a recombinant inbred line (RIL) population. A total of 166 markers, consisting of SSRs, RAPDs, STSs, and SRAPs were developed for the mapping population. Twenty-eight linkage groups were constructed from 142 markers. Comparative marker mapping between F2 and RIL populations indicated a linkage map expansion in the RIL population, which strongly supports the strategy to develop the RIL population for more accurate genetic mapping. Other 24 markers that are polymorphic between the two parents did not segregate in the RIL population, indicating selective elimination on the related chromosomal regions.
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