Batman P, Fleming SC, MacDonald T, Sedgwick P, Griffin GE; International Conference on AIDS.

Int Conf AIDS. 1992 Jul 19-24; 8: A16 (abstract no. PoA 2080).

St. George's Hospital Medical School, London, UK.

OBJECTIVE: To quantitate crypt cell proliferation in human foetal intestinal mucosal explants maintained and infected with HIV in organ culture. METHOD: 20 human foetal jejunal and colonic explants, obtained at 16 weeks gestational age, were cultured in vitro. Each piece of tissue was dissected into 2mm3 aliquots for organ culture and two parallel cultures consisting of six explants were cultured in vitro for each tissue specimen. One culture was infected with HIV (IIIB), by incubating with virus for 30 minutes in the presence of DEAE Dextran and repeated washing, and the other served as non-infected control. Explants were harvested at 4, 7, 10 or 14 days (t) post infection, fixed in formalin, embedded in paraffin wax, and sections stained by indirect immunoperoxidase method to proliferating cell nuclear antigen (DAKO). Stained sections were examined with a light microscope, and the total number of crypt cells in proliferation expressed as percentage of the total number of crypt cells (greater than 300 counted per explant) in the control (C) and HIV-infected (H) explant of each pair. The difference between the two values for each pair was called the crypt proliferation index (CPI) (positive CPI: H greater than C; negative CPI C greater than H). RESULTS: 15 pairs showed a progressively positive CPI with time (sign test p less than 0.02) indicating crypt hyperplasia; 3 of 5 at t4, median 13.3 semi interquartile range (SIQ) 20.6; 4 of 5 at t7, median 16.1, SIQ 3.6; 4 of 6 at t10, median 14.9, SIQ 13.2; 4 at t14, median 54.5, SIQ 20.5. One pair showed zero difference and four pairs showed negative CPI. CONCLUSION: HIV induces crypt cell proliferation in human foetal mucosal explants maintained in vitro and may represent a model for jejunal crypt hyperplasia previously described in vivo.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Animals
• Colon
• Fetus
• HIV
• HIV Core Protein p24
• HIV Envelope Protein gp41
• HIV Infections
• HIV Seropositivity
• Humans
• Hyperplasia
• In Vitro
• Intestinal Mucosa
• Intestines
• Jejunum
• Organ Culture Techniques
• immunology
• reverse transcriptase, Human immunodeficiency virus 1

Other ID:

• 92400373

UI: 102198086

From Meeting Abstracts