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Library Info Page
| Library Name: | Soares_NMEBA_branchial_arch |
| Organism: | Mus musculus |
Strain: | |
| UniGene Library ID: | 5417 |
| Tissue Description: | Branchial Arches, mouse |
| Library Keywords: | normal, bulk, normalized, CGAP, EST, Soares normalized, branchial arch, phagemid, oligo-dT primed, organogenesis |
| #Clones Generated to Date: | 8832 |
| #Sequences Generated to Date: | 7956 |
| Library Preparation Details |
| Description: | 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATGCATTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo. |
| R. Site1: | NotI |
| R. Site2: | EcoRI |
| Lab Host: | DH10B (phage resistant) |
| Vector: | pT7T3D-PacI |
| Vector Type: | phagemid (ampicillin resistant) |
| Tissue Supplier: | M. Bento Soares, Ph.D. |
| Library Producer: | Soares Laboratory |
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