TM 157 Table B26

Table B26a. Analyses (ng/g [ppb] wet weight) of replicates of NIST mussel tissue V (QA93TIS5)^a for determination of the method detection limit (MDL) for low-molecular-weight PAHs
	Naph- thalene	2-methyl- naph- thalene	1-methyl- naph- thalene	Biphenyl	2,6-di- methyl- naph- thalene	Acenaph- thylene	Acenaph- thene	2,3,5-tri- methyl- naph- thalene	Fluorene	Phen- anthrene	Anthra- cene	1-methyl- phen- anthrene
Replicate 1	nf^b	nf^b	nf^b	nf^b	nf^b	1.34	nf^b	nf^b	nf^b	1.48	2.14	1.91
Replicate 2	1.03	1.07	0.81	0.89	1.03	0.91	0.81	nf^b	nf^b	1.84	1.33	2.02
Replicate 3	0.500	1.05	0.85	1.44	nf^b	0.91	1.36	nf^b	nf^b	2.22	nf^b	2.36
Replicate 4	0.43	1.13	0.36	0.99	1.44	nf^b	1.09	nf^b	nf^b	2.31	nf^b	nf^b
Replicate 5	0.53	0.880	nf^b	0.98	nf^b	nf^b	nf^b	nf^b	nf^b	2.28	nf^b	nf^b
Replicate 6	0.66	0.78	0.81	1.39	nf^b	nf^b	1.92	nf^b	nf^b	2.57	nf^b	nf^b
Replicate 7	2.44	1.91	2.06	2.49	nf^b	2.49	nf^b	nf^b	nf^b	5.16	nf^b	nf^b
Replicate 8	nf^b	0.47	nf^b	0.27	nf^b	nf^b	nf^b	nf^b	nf^b	1.70	1.07	1.74
n =	6	7	5	7	2	4	4	-	-	8	3	4
Mean	0.933	1.04	0.979	1.21	1.23	1.41	1.29	-	-	2.44	1.51	2.01
Std. dev.	0.771	0.444	0.636	0.686	0.288	0.746	0.473	-	-	1.15	0.555	0.261
RSD (%)	83	43	65	57	23	53	37	-	-	47	37	13
Student's "t"	3.36	3.14	3.75	3.14	31.8	4.54	4.54	-	-	3	6.96	4.54

MDL^c	2.59	1.40	2.38	2.16	9.18	3.39	2.15	10 ^b	10 ^b	3.46	3.87	1.19
^aNIST mussel tissue V (QA93TIS5) was included in each of eight extraction batches as a part of the QA/QC protocol. ^bnf = peak not found; assuming 10 g wet weight of mussel tissue, 50% efficiency in the sample extraction and cleanup steps, a 250L final sample colume, and an instrument (GC/MS) detection limit of 0.2 ng/L, the MDL calculates to be 10 ppb wet weight. ^cMDL t, where = standard deviation and t = Student's "t" value with n-1 degrees of freedom and = 0.01 (one tailed).

Table B26b. Analyses (ng/g [ppb] wet weight) of replicates of NIST mussel tissue V (QA93TIS5)^a for determination of the method detection limit (MDL) for high-molecular-weight PAHs
	Fluor- anthene	Pyrene	Benz[a]- anthra- cene	Chrysene	Benzo[b]- fluoran- thene	Benzo[k]- fluoran- thene	Benzo[e]- pyrene	Benzo[a]- pyrene	Pery- lene	Indeno- [1,2,3- cd] pyrene	Dibenz- [a,h]- anthra- cene	Benzo- [ghi]- perylene
Replicate 1	14.3	14.0	7.81	12.22	8.81	3.88	11.04	1.96	nf^b	1.37	nf^b	1.17
Replicate 2	13.0	12.2	6.48	10.53	6.94	3.39	8.32	1.76	nf^b	0.928	nf^b	0.675
Replicate 3	13.2	12.1	7.00	9.53	7.63	5.68	8.41	4.84	nf^a	5.73	nf^a	5.64
Replicate 4	13.8	12.7	7.75	10.92	8.48	3.77	10.78	2.37	1.66	nf^b	nf^b	nf^b
Replicate 5	12.6	12.0	7.80	10.45	6.87	3.57	8.52	nf^b	nf^b	nf^b	nf^b	nf^b
Replicate 6	10.7	10.5	6.13	8.58	5.73	2.88	7.67	1.74	nf^b	nf^b	nf^b	3.36
Replicate 7	11.6	10.8	7.15	8.47	6.70	5.48	7.21	4.98	nf^b	nf^b	nf^b	nf^b
Replicate 8	15.2	15.8	5.62	8.72	4.24	2.77	4.18	nf^b	nf^b	nf^b	nf^b	nf^b
n =	8	8	8	8	8	8	8	6	-	3	-	4
Mean	13.0	12.5	6.97	9.93	6.92	3.93	8.27	2.94	-	2.68	-	2.71
Std. dev.	1.44	1.71	0.828	1.33	1.47	1.09	2.15	1.54	-	2.65	-	2.27
RSD (%)	11	14	12	13	21	28	26	52	-	99	-	84
Student's "t"	3	3	3	2.998	3	3	2.998	3.36	-	6.7	-	4.54

MDL^c	4.33	5.12	2.48	3.99	4.41	3.28	6.44	5.19	10^b	17.8	10^b	10.3
^aNIST mussel tissue V (QA93TIS5) was included in each of eight extraction batches as a part of the QA/QC protocol. ^bnf = peak not found; assuming 10 g wet weight of mussel tissue, 50% efficiency in the sample extraction and cleanup steps, a 250L final sample colume, and an instrument (GC/MS) detection limit of 0.2 ng/L, the MDL calculates to be 10 ppb wet weight. ^cMDL t, where = standard deviation and t = Student's "t" value with n-1 degrees of freedom and = 0.01 (one tailed).

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(Modified Jun. 13 2008)