| CRD 03-11
Table 1. A. anophagefferens enumeration (cells ml -1)
of Long Island, NY, samples at the James J. Howard Marine Sciences Laboratory
(HL) following various disaggregation treatments. Results after process
with all disaggregation treatments combined are compared with prior enumeration
by the Suffolk County, NY, Department of Health Services (SCDHS). SCDHS
processed samples collected on 12-2-99 after 50 days storage and samples
collected on12-29-99 after 23 days storage. HL reprocessed these after
84-136 days storage. Treatments: Vort : vortexing; Pyro: sodium pyrophosphate;
Son: sonication. A minimal amount of aggregated cells was observed in two
of the Vort/Pyro/Son-treated samples: three clumps of 2-3 cells in one
and an embed of 6 cells in another. |
Test
No. |
Station
No. |
Date
Collected |
Vort |
Vort/
Pyro |
Vort
/Pyro
/Son |
SCDHS
enumeration |
HL all
treatments/
SCDHS,
% +,- |
1 |
090110 |
12-2-99 |
40,900 |
52,867 |
223,398 |
285,950 |
-22 |
2 |
090130 |
12-2-99 |
59,946 |
71,203 |
461,862 |
499,732 |
-8 |
3 |
090160 |
12-2-99 |
99,856 |
221,674 |
459,235 |
521,518 |
-22 |
4 |
090200 |
12-29-99 |
7,023 |
13,583 |
21,506 |
18,723 |
14 |
5 |
090280 |
12-29-99 |
6,099 |
199,599 |
204,625 |
166,804 |
22 |
|
6 |
090120 |
12-2-99 |
102,682 |
112,280 |
438,246 |
311,481 |
40 |
7 |
090280 |
12-2-99 |
57,597 |
199,599 |
336,440 |
228,760 |
47 |
8 |
090110 |
12-29-99 |
47,268 |
231,172 |
318,814 |
236,930 |
34 |
|
9 |
090190 |
12-2-99 |
48,187 |
333,663 |
321,648 |
150,494 |
113 |
10 |
090150 |
12-2-99 |
119,711 |
184,241 |
665,981 |
328,842 |
102 |
Table 2. Effect of 1.0% AQUET detergent combined with
vortexing (30 sec.), pyrophosphate (0.0001M, 15 min.) and varied sonication
time on enumeration of A. anophagefferens (cells ml-1)
in aliquots of the same sample. Microscope observations of cell aggregates
in the preparations are noted. |
| |
Treatment |
Treatment |
Vort/
Son/
Pyro |
Presence
of cell
aggregates |
Vort/
Son/
Pyro/
Aquet |
Presence
of cell
aggregates |
Test
No. |
Son time
(sec.) |
|
| 1 |
30 |
64,636 |
multiple embeds |
242,514 |
none |
| 2 |
40 |
53,988 |
" |
225,036 |
one embed/one clump |
| 3 |
50 |
37,825 |
" |
193,586 |
one embed |
| 4 |
60 |
26,127 |
" |
270,292 |
none |
| 5 |
70 |
64,378 |
" |
235,766 |
three embeds |
| 6 |
80 |
96,068 |
" |
215,910 |
single embed |
| 7 |
90 |
206,798 |
single embed |
248,848 |
single embed |
| Table 3. Enumeration changes for representative A. anophagefferens bloom
initiation and development samples following full disaggregation protocol
treatment. Microscope scan for cell aggregates showed: none (none); many
clumps (clmp-m); few clumps (clmp-f). "R"signifies processing repeated,
with sample dilution and additional mixing, soon after initial processing.
If a sample was "R" reprocessed the highest count obtained was used for
the ratio calculation. |
Sample
No. |
Initial
process
date |
Initial
population
estimate |
Presence
of cell
aggregates |
Reprocess
date/post
treatment |
Revised
population
estimate |
Presence
of cell
aggregates |
Ratio of revised to
initial population
estimates |
| Bloom initiation: |
| 544 |
4-8-99 |
214 |
none |
11-29-00 |
752 |
none |
3.5 |
| 546 |
4-8-99 |
107 |
none |
11-29-00 |
1,613 |
none |
15 |
| Bloom development: |
| 592 |
5-19-99 |
129,160 |
clmp-m |
8-27-01 |
2.3x106 |
clmp-f |
17.8 |
| 594 |
5-19-99 |
274,656 |
clmp-f |
8-27-01 |
787,169 |
none |
2.8 |
| 606 |
5-26-99 |
130,218 |
clmp-f |
|
| 606R |
7-26-99 |
102,251 |
clmp-f |
1-16-02 |
276,434 |
none |
2.1 |
| 643 |
6-30-99 |
270,278 |
clmp-m |
|
| 643R |
7-5-99 |
1.6x106 |
clmp-f |
5-1-01 |
1.9x106 |
clmp-f |
1.2 |
| 645 |
6-10-99 |
81,084 |
clmp-f |
11-15-01 |
796,976 |
none |
9.8 |
| 660 |
8-2-99 |
47,051 |
clmp-m |
| 660R |
8-4-99 |
85,926 |
clmp-m |
4-25-02 |
2.8x106 |
clmp-f |
35.6 |
| 661 |
8-2-99 |
16,242 |
none |
|
| 661R |
8-9-99 |
11,668 |
none |
12-28-00 |
29,089 |
none |
1.8 |
| 677 |
6-30-99 |
322,900 |
clmp-m |
| 677R |
7-5-99 |
2.6x106 |
clmp-f |
8-8-01 |
4.2x106 |
none |
1.6 |
| 678 |
6-30-99 |
444,489 |
clmp-m |
8-8-01 |
3.8x106 |
none |
8.5 |
| 683 |
6-23-99 |
15,068 |
clmp-f |
8-8-01 |
201,812 |
none |
13.4 |
|