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Tools for Insertional Mutagenesis in the Mouse
Release Date: January 25, 2001
Announcement Number: RFA-DA-01-011
Application Receipt Date:
April 11, 2001
Letter of Intent Date: March 11, 2001
Funding Contact: NINDS Funding
Program Area: Neurogenetics
Brief Description:
This RFA solicits proposals for development of tools and techniques for the establishment of random and targeted sequence-tagged
insertion libraries of embryonic stem (ES) cells that can be used to generate mutant mice in which the expression of the tagged
gene could be controlled temporally and spatially. The development of such a resource for wide distribution to the scientific
community would make it possible to scan the sequence database for any gene of interest and order the corresponding targeted
ES cell line. Ideally, the insertional mutagenesis system developed would permit a wide range of genetic analyses and manipulations,
including enhancer-trapping, conditional knockouts, conditional expression or overexpression, etc. It also would permit the
larger community of investigators to utilize genomic resources efficiently.
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