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Library Info Page
| Library Name: | NCI_CGAP_PlTr1 |
| Organism: | Mus musculus |
Strain: | C57BL/6J |
| UniGene Library ID: | 10407 |
| Tissue Description: | Placenta, trophoblast, mouse, derived from NCI_CGAP_Pl8, NCI_CGAP_Pl9, NCI_CGAP_Tr1 |
| Library Keywords: | normal, bulk, pregnant, adult, subtracted, CGAP, EST, Soares subtracted, female, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6J, trophoblast |
| #Clones Generated to Date: | |
| #Sequences Generated to Date: | 5721 |
| Library Preparation Details |
| Description: | NCI_CGAP_PlTr1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GTGTG, ATCAT, GGGTG, GGTTG. For additional information, contact: Bento Soares, bento-soares@uiowa.edu |
| R. Site1: | EcoR I |
| R. Site2: | Not I |
| Lab Host: | DH10B (Life Technologies) |
| Vector: | pT7T3-Pac (Pharmacia) with a modified polylinker |
| Vector Type: | Plasmid |
| Tissue Supplier: | Dr. Vera M. da Costa Soares, Dr. Minoru Ko, Dr. Tilo Kunath |
| Library Producer: | Soares Laboratory |
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