Testing Status of Agents at NTP
National Toxicology Program (NTP) Testing Information
Asphalt Table B-3
APPENDIX B
SUMMARY OF ANIMAL DATA
Table B-3. Skin-painting data for the interscapular region of mice [Sivak et al. 1989]
| Species and substance* tested | Number mice with carcinomas | Comments |
|---|---|---|
| C3H/HeJ mice: | The fume condensates were separated into 5 fractions and tested individually and in combination with each other along with raw asphalt and neat asphalt fume. | |
| Asphalt: | ||
| Raw asphalt | 3 | |
| Heated asphalt | 0 | |
| Heated asphalt & fume | 0 | |
| Neat asphalt fume | 20 | |
| Solvent control | 0 | |
| Asphalt Fractions: | ||
| Fraction A | 0 | |
| Fraction B | 10 | |
| Fraction C | 17 | |
| Fraction D | 0 | |
| Fraction E | 0 | |
| Fractions A,B,C,D,E | 19 | |
| Fractions A,B | 8 | |
| Fractions A,C | 11 | |
| Fractions A,D | 0 | |
| Fractions A,E | 0 | |
| Fractions B,C,D,E | 15 | |
| Fractions A,B,C,D | 18 | |
| Fractions A,B,C,E | 21 | |
| Fractions B,C,D | 15 | |
| Fractions B,C | 24 | |
| Fractions A,C,D,E | 14 | |
| Fractions A,B,D,E | 7 | |
| Fractions A,D,E | 0 | |
| BaP only: | ||
| 0.01% BaP | 27 | |
| 0.001% BaP | 3 | |
| 0.0001% BaP | 0 | |
| Asphalt Fraction + BaP: | Cocarcinogenicity of fractions A,D,E were tested with 0.01%, 0.001%, and 0.0001% BaP | |
| FractionA+0.01%BaP | 23 | |
| Fraction A+ 0.001% BaP | 1 | |
| Fraction A + 0.0001% BaP | 1 | |
| Fraction D + 0.01% BaP | 25 | |
| Fraction D + 0.001% BaP | ||
| Fraction D + 0.0001% BaP | 1 | |
| Fraction E + 0.01% BaP | 23 | |
| Fraction E + 0.001% BaP | 2 | |
| Fraction E + 0.0001% BaP | 0 | |
| 200 mg BaP + fraction: | Single treatment of 200 mg of BaP followed by 50-ml treatments of fractions A,Dm, & E were used to test for tumor-promoting activity. | |
| 200 mg BaP then A | 0 | |
| 200 mg BaP then D | 0 | |
| 200 mg BaP then E | 0 | |
| 200 mg BaP alone | 0 | |
| Sentinel mice† | 0 | |
| Sencar mice: | ||
| Fume | 14 | |
| Control | 0 | |
* This study used Type III roofing asphalt condensates generated at 316°C.
† Group used for monitoring the health of the C3H/HeJ mice during the course of the study.
Web page last updated on August 15, 2005
