Projects

DETERMINANTS OF AVIAN COCCIDIOSIS INFECTION AND PATHOGENICITY Appropriated (D)   Accession Number: 412453

PROTOZOAN PARASITES AFFECTING FOOD ANIMALS, FOOD SAFETY, AND PUBLIC HEALTH Appropriated (D)   Accession Number: 409819

EPIDEMIOLOGY AND CONTROL OF NEOSPORA CANINUM AND RELATED PROTOZOA Appropriated (D)   Accession Number: 411466

Publications

Cloning, expression, and characterization of Giardia duodenalis, beta-giardin - (Abstract)

Trout, J.M., Obrien, C.N., Miska, K.B., Schwarz, R.S., Jenkins, M.C. Cloning, expression, and characterization of Giardia duodenalis, beta-giardin. The American Association of Veterinary Parasitologists (AAVP) 53rd Annual Meeting, July 19-22, 2008, New Orleans, LA. No. 57.

Application of RT-PCR to study in vitro development of Cryptosporidium parvum and its viral symbiont CPV - (Abstract)

Jenkins, M.C., Obrien, C.N., Rosenthal, B.M., Fayer, R. 2008. Application of RT-PCR to study in vitro development of Cryptosporidium parvum and its viral symbiont CPV. Annual Meeting of American Society of Parsitologists, Arlington, Texas June 26-30, 2008.

EIMERIA PRAECOX INFECTION AMELIORATES EFFECTS OF E. MAXIMA INFECTION IN CHICKENS - (Peer Reviewed Journal)

Jenkins, M.C., Allen, P., Wilkins, G., Klopp, S., Miska, K.B. 2008. Eimeria praecox infection ameliorates effects of e. maxima infection in chickens. 155:10-14.

Molecular and morphological characterization of Eimeria in game birds. - (Abstract)

Miska, K.B., Schwarz, R.S., Macfarlane, B., Wallner-Pendleton, Jenkins, M.C. 2008. Molecular and morphological characterization of Eimeria in game birds. American Society of Parasitologists. p108.

Fecundity of Cryptosporidium parvum is Correlated with Intracellular Levels of the Viral Symbiont CPV - (Peer Reviewed Journal)

Jenkins, M.C., Higgins, J.A., Brahante, J., Kniel, K., Obrien, C.N., Trout, J.M., Lancto, C., Abrahamsen, M., Fayer, R. 2008. Fecundity of Cryptosporidium parvum is Correlated with Intracellular Levels of the Viral Symbiont CPV. International Journal for Parasitology. 38:1051-1055.

Neosporosis in Beagle dogs clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum. - (Peer Reviewed Journal)

Dubey, J.P., Vianna M, C.R., Kwok, O.C., Hill, D.E., Miska, K.B., Tuo, W., Velmurugan, G.V., Conors, M., Jenkins, M.C. 2007. Neosporosis in Beagle dogs clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum. Veterinary Parasitology. 149:158-166.

Detection of Cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to CPV40 capsid protein - (Peer Reviewed Journal)

Jenkins, M.C., Obrien, C.N., Trout, J.M. 2008. Detection of Cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to CPV40 capsid protein. Journal of Parasitology. 94:94-98.

The evaluation of ISCOMs incorporating triterpene saponins isolated from native plants and the recombinant antigen SO7 for in ovo immunization of chickens against Eimeria tenella infection. - (Abstract)

Barfield, R.C., Miska, K.B., Jenkins, M.C., Fetterer, R.H., Berezin, V., Bogoyavlenskiy, A., Tolmacheva, V., Makhmudova, N., Khudyakoval, S., Levandovskaya, S., Omirtaeva, E., Zautceva, I., Tustikbaeva, G., Aleksyuk, P. 2007. The evaluation of ISCOMs incorporating triterpene saponins isolated from native plants and the recombinant antigen SO7 for in ovo immunization of chickens against Eimeria tenella infection. American Association of Veterinary Parasitologists. p66.

Intracellular levels of the viral symbiont CPV in Cryptosporidium parvum correlate with fecundity of the parasite in dairy calves - (Abstract)

Higgins, J.A., Obrien, C.N., Abrahante, J., Abrahamsen, M., Fayer, R., Kniel, K., Jenkins, M.C. 2007. Intracellular levels of the viral symbiont CPV in Cryptosporidium parvum correlate with fecundity of the parasite in dairy calves. American Association of Veterinary Parasitologists, July 14-18, 2007, Washington, D.C.

Characterization of the antigen SO7 during development of Eimeria tenella. - (Peer Reviewed Journal)

Fetterer, R.H., Jenkins, M.C., Miska, K.B., Barfield, R.C. 2007. Characterization of the antigen SO7 during development of Eimeria tenella. Journal of Parasitology. 93:1107-1113.

Characterization of novel lytic peptide secreted by intestinal intraepithelial lymphocytes infected with coccidia - (Proceedings/Symposium)

Lillehoj, H.S., Lee, S.H, Jenkins, M.C., Hong, Y.H. 2007. Characterization of novel lytic peptide secreted by intestinal intraepithelial lymphocytes infected with coccidia. Proceedings of American Avian Veterinary Pathologists meeting. July 14-18. Washington, D.C.

HIGH HYDROSTATIC PRESSURE AND UV LIGHT TREATMENT OF PRODUCE CONTAMINATED WITH EIMERIA ACERVULINA AS A CYCLOSPORA CAYETANENSIS SURROGATE - (Peer Reviewed Journal)

Kniel, K., Shearer, A., Cascarino, J., Wilkins, G., Jenkins, M.C. 2007. High hydrostatic pressure and uv light treatment of produce contaminated with eimeria acervulina as a cyclospora cayetanensis surrogate. Journal of Food Protection. 70:2837-2842.

Effects of high hydrostatis pressure on Eimeria Acervulina pathogenicity, immunogenicity and structural integrity. - (Peer Reviewed Journal)

Shearer, A.H., Wilkins, G.C., Jenkins, M.C., Kniel, K.E. 2007. Effects of high hydrostatis pressure on Eimeria acervulina pathogenicity, immunogenicity and structural integrity. Innovative Food Science and Emerging Technologies. 8:259-268.

A recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine encoding Eimeria acervulina antigen offers protection against E. acervulina challenge. - (Peer Reviewed Journal)

Konjufca, V., Wanda, S., Jenkins, M.C., Curtiss, Iii, R. 2006. A recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine encoding Eimeria acervulina antigen offers protection against E. acervulina challenge. Infection and Immunity. 74:6785-6796.

A RAPID METHOD FOR PRODUCTION OF HIGH NUMBERS OF PURIFIED CRYPTOSPORIDIUM PARVUM OOCYSTS - (Peer Reviewed Journal)

Obrien, C.N., Jenkins, M.C. 2007. A rapid method for production of high numbers of purified Cryptosporidium parvum oocysts. Journal of Parasitology. 93:434-436.

NEOSPORA CANINUM DETECTED IN WILD RODENTS - (Peer Reviewed Journal)

Jenkins, M.C., Parker, C.C., Hill, D.E., Pinckney, R.D., Dubey, J.P. 2007. Neospora caninum detected in wild rodents. Veterinary Parasitology. 143:161-165.

THE FIRST CHARACTERIZATION OF MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) MOLECULES FROM APICOMPLEXAN PARASITES BELONGING TO GENUS EIMERIA, THE CAUSATIVE AGENTS OF CHICKEN COCCIDIOSIS. - (Peer Reviewed Journal)

Miska, K.B., Fetterer, R.H., Lillehoj, H.S., Jenkins, M.C., Allen, P.C., Harper, S. 2007. The first characterization of macrophage migration inhibitory factor (mif) molecules from apicomplexan parasites belonging to genus Eimeria, the causative agents of chicken coccidiosis. Molecular and Biochemical Parasitology. 151:173-183.

Comparison of serological methods for the diagnosis of Neospora caninum infection in cattle - (Peer Reviewed Journal)

Wapenaar, W., Barkema, H.W., Van Leeuwen, J.A., Mc Clure, J.T., O'Handley, R.M., Kwok, O.C., Thulliez, P., Dubey, J.P., Jenkins, M.C. 2007. Comparison of serological methods for the diagnosis of Neospora caninum infection in cattle. Veterinary Parasitology. 143:166-173.

AN IMPROVED POLYMERASE CHAIN REACTION TECHNIQUE FOR DETERMINING THE SPECIES COMPOSITION OF EIMERIA IN POULTRY LITTER - (Peer Reviewed Journal)

Jenkins, M.C., Miska, K.B., Klopp, S. 2006. An improved polymerase chain reaction technique for determining the species composition of Eimeria in poultry litter. Avian Diseases. 50:632-635.

APPLICATION OF POLYMERASE CHAIN REACTION BASED ON ITS1 RDNA TO SPECIATE EIMERIA - (Peer Reviewed Journal)

Jenkins, M.C., Miska, K.B., Klopp, S. 2006. Application of polymerase chain reaction based on its1 rdna to speciate eimeria. Avian Diseases. 50:110-114.

ACETYL COA CARBOXYLASE IN AVIAN EIMERIA - (Abstract)

Allen, P.C., Miska, K.B., Jenkins, M.C., Fetterer, R.H. 2005. Acetyl coa carboxylase in avian eimeria. Coccidiosis International Conference Proceedings. p. 164.

THE MACROPHAGE INHIBITORY FACTOR OF EIMERIA SPECIES - (Abstract)

Miska, K.B., Lillehoj, H.S., Allen, P.C., Fetterer, R.H., Jenkins, M.C. 2005. The macrophage inhibitory factor of eimeria species. Coccidiosis International Conference Proceedings. p. 163.

COMPARISON OF DRUG-SENSITIVITY AND SPECIES COMPOSITION OF EIMERIA ISOLATED FROM POULTRY FARMS UTILIZING EITHER ANTI-COCCIDIAL DRUGS OR A LIVE OOCYST VACCINE TO CONTROL AVIAN COCCIDIOSIS. - (Abstract)

Jenkins, M.C., Klopp, S., Wilkins, G.C., Miska, K.B. 2005. Comparison of drug-sensitivity and species composition of eimeria isolated from poultry farms utilizing either anti-coccidial drugs or a live oocyst vaccine to control avian coccidiosis. Coccidiosis International Conference Proceedings. p.42

NEOSPORA CANINUM CYCLOPHILIN: A INTERFERON-GAMMA-INDUCING PROTEIN THAT MAY MEDIATE THE PARASITE-HOST INTERACTIONS. - (Abstract)

Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2005. Neospora caninum cyclophilin: a interferon-gamma-inducing protein that may mediate the parasite-host interactions.. Meeting Abstract.

NEOSPORA CANINUM IMMUNOPHILIN STIMULATES INTERFERON-GAMMA PRODUCTION - (Abstract)

Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2005. Neospora caninum immunophilin stimulates interferon-gamma production. Meeting Abstract.

HPLC PURIFICATION OF RECOMBINANT NCGRA6 ANTIGEN IMPROVES ENZYME-LINKED IMMUNOSORBENT ASSAY FOR SERODIAGNOSIS OF BOVINE NEOSPOROSIS - (Peer Reviewed Journal)

Jenkins, M.C., Fetterer, R.H., Schares, G., Bjorkman, C., Mcallister, M., Dubey, J.P. 2005. Hplc purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis. Veterinary Parasitology. 131:227-234.

CROSS PROTECTION STUDIES WITH EIMERIA MAXIMA STRAINS - (Peer Reviewed Journal)

Allen, P.C., Jenkins, M.C., Miska, K.B. 2005. Cross protection studies with eimeria maxima strains. Parasitology Research. 97:179-185

IDENTIFICATION AND CHARACTERIZATION OF NEOSPORA CANINUM CYCLOPHILIN THAT ELICITS INTERFERON-GAMMA PRODUCTION - (Peer Reviewed Journal)

Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2005. Identification and characterization of Neospora caninum cyclophilin that elicits interferon-gamma production. Infection and Immunity. 73(8):5093-5100.

IMPROVING THE SPECIFICITY OF 16S-RDNA-BASED POLYMERASE CHAIN REACTION FOR DETECTING BORRELIA BURGDOFERI SENSU LATO-CAUSATIVE AGENTS OF HUMAN LYME DISEASE. - (Peer Reviewed Journal)

Cyr, T.L., Jenkins, M.C., Gilborn, A., Masters, E.J., Mcdonald, G.A. 2005. Improving the specificity of 16s-rDNA-based polymerase chain reaction for detecting Borrelia burgdoferi sensu lato-causative agents of human Lyme disease. Journal of Applied Microbiology. 98:962-970.

DIAGNOSIS OF PARASITIC INFECTIONS - (Abstract)

Jenkins, M.C. 2004. Diagnosis of parasitic infections. Proceedings DU COST Meeting on Neusporosis, p. 14

USE OF RECOMBINANT ANTIGEN IN SERODIAGNOSIS OF PROTOZOAN INFECTION: ADVANTAGES AND LIMITATIONS IN DIAGNOSING BOVINE NEOSPOROSIS - (Abstract)

Jenkins, M.C. 2004. Use of recombinant antigen in serodiagnosis of protozoan infection: advantages and limitations in diagnosing bovine neosporosis. Meeting Abstract.

NEOSPORA CANINUM ANTIGEN DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS - (Peer Reviewed Journal)

Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2005. Neospora caninum antigen defined by antigen-dependent bovine cd4+ t cells. Journal of Parasitology 91(3):564-568.

BIOLOGIC, MORPHOLOGIC AND MOLECULAR CHARACTERIZATION OF NEOSPORA CANINUM ISOLATES FROM LITTERMATE DOGS - (Abstract)

Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M., Kwok, O.C., Hill, D.E., Jenkins, M.C., Lindsay, D.S., Greene, C.E. 2004. Biologic, morphologic and molecular characterization of neospora caninum isolates from littermate dogs (Abstract). COST 454 Annual Conference, Protozoal Reproduction Losses in Farm Ruminants. p.1.

VACCINATION OF SHEEP WITH NEOSPORA CANINUM PROTEIN PRODUCTS AGAINST FETAL LOSS ASSOCIATED WITH EXPERIMENTAL NEOSPOROSIS - (Peer Reviewed Journal)

Jenkins, M.C., Tuo, W., Dubey, J.P. 2004. Vaccination of sheep with neospora caninum protein products against fetal loss associated with experimental neosporosis. American Journal of Veterinary Research 65:1404-1408.

IMPROVING THE SPECIFICITY OF 16S-RDNA-BASED POLYMERASE CHAIN REACTION FOR DETECTING BORRELLA BURGDORFERI SENSU LATO-CAUSATIVE AGENTS OF HUMAN LYME DISEASE. - (Peer Reviewed Journal)

Cyr, T.L., Jenkins, M.C., Hall, R.D., Masters, E.J., Mcdonald, G.A. 2004. Improving the specificity of 16s-rdna-based polymerase chain reaction for detecting Borrella burgdorferi sensu lato-causative agents of human lyme disease. Journal of Microbiological Methods. 98:962-970.

CHARACTERIZATION AND POTENTIAL USE OF A CRYPTOSPORIDIUM PARVUM VIRUS (CPV) ANTIGEN FOR DETECTING C. PARVUM OOCYSTS. - (Peer Reviewed Journal)

Kniel, K.E., Higgins, J.A., Trout, J.M., Fayer, R., Jenkins, M.C. (2004) Characterization and potential use of a Cryptosporidium parvum virus (CPV) antigen for detecting C. parvum oocysts. Journal of Microbiological Methods 58: 189-195.

PROTECTION OF CALVES AGAINST CRYPTOSPORIOSIS BY ORAL INOCULATION WITH GAMMA-IRRADIATED CRYPTOSPORIDIUM PARVUM OOCYSTS. - (Peer Reviewed Journal)

Jenkins, M.C., Higgins, J.A., Kniel, K., Trout, J.M., Fayer, R. (2004) Protection of calves against cryptosporidiosis by oral inoculation with gamma-irradiated Cryptosporidium parvum oocysts. Journal of Parasitology. 90: 830-833.

CLINICAL, BIOLOGIC, MORPHOLOGIC, AND MOLECULAR CHARACTERISATION OF NEOSPORA CANINUM INFECTIONS IN LITTERMATE DOGS - (Peer Reviewed Journal)

Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M.C., Kwok, O.C., Hill, D.E., Jenkins, M.C., Lindsay, D.S., Greene, C.E. 2004. Clinical, biologic, morphologic, and molecular characterisation of neospora caninum infections in littermate dogs. International Journal for Parasitology 34:1157-1167.

CONTROL OF AVIAN COCCIDIOSIS: DRUGS AND VACCINES - (Review Article)

Jenkins, M.C. 2004. Control of avian coccidiosis: drugs and vaccines. Miscellaneous Publishing Information Bulletin. Feed Information News Service, p. 3.

PROGRESS ON DEVELOPING A NATIVE RECOMBINANT VACCINE AGAINST BOVINE NEOSPOROSIS - (Abstract)

Jenkins, M.C., Tuo, W., Dubey, J.P. 2004. Progress on developing a native recombinant vaccine against bovine neosporosis. Annual Meeting of American Society of Parasitologists, p. 84.

A CONSERVED 19 KDA EIMERIA TENELLA ANTIGEN IS A PROFILIN-LIKE PROTEIN - (Peer Reviewed Journal)

Fetterer, R.H., Miska, K.B., Jenkins, M.C., Barfield, R.C. 2004. A conserved 19 kda Eimeria tenella antigen is a profilin-like protein. Journal of Parasitology. 90:1321-1328.

IN SEARCH OF IMMUNODOMINANT NEOSPORA CANINUM ANTIGENS USING IMMUNOLOGIC AND PROTEOMICS TECHNOLOGIES - (Abstract)

Tuo, W., Fetterer, R.H., Davis, B., Jenkins, M.C., Dubey, J.P. 2005. In search of immunodominant neospora caninum antigens using immunologic and proteomics technologies. 15th Annual BARC Poster Day, p. 51.

FUNCTIONAL CHARACTERIZATION OF HPLC-FRACTIONATED NEOSPORA CANINUM PROTEINS. - (Abstract)

Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2004. Functional characterization of hplc-fractionated neospora caninum proteins. American Association of Veterinary Parasitologists p. 123.

ESTABLISHMENT OF SHORT-TERM CD4+ T CELL LINES RECOGNIZING NEOSPORA CANINUM ANTIGEN - (Peer Reviewed Journal)

Tuo, W., Davis, W., Fetterer, R.H., Jenkins, M.C., Boyd, P., Gasbarre, L.C., Dubey, J.P. 2003. Establishment of short-term cd4+ T cell lines recognizing Neospora caninum antigen. Parasite Immunology. 26:243-246.

LOCALIZATION OF A 56 KDA ANTIGEN THAT IS PRESENT IN MULTIPLE DEVELOPMENTAL STAGES OF NEOSPORA CANINUM - (Peer Reviewed Journal)

Jenkins, M.C., Soares, R., Murphy, C.A., Hemphill, A., O'Handley, R., Dubey, J.P. 2004. Localization of a 56 kda antigen that is present in multiple developmental stages of neospora caninum. Journal of Parasitology. 90:660-663.

INCLUSION OF CPG ADJUVANT WITH PLASMID DNA CODING FOR NCGRA7 IMPROVES PROTECTION AGAINST CONGENITAL NEOSPOROSIS - (Peer Reviewed Journal)

Jenkins, M.C., Parker, C.C., Tuo, W., Vinyard, B., Dubey, J.P. 2004. Inclusion of cpg adjuvant with plasmid dna coding for ncgra7 improves protection against congenital neosporosis. Infection and Immunity. 72:1817-1819.

ARE THERE IMMUNOVARIANTS OF COCCIDIA THAT CAN AFFECT VACCINE EFFICACY? (THE CLUES MAY NOT ALWAYS BE OBVIOUS) - (Peer Reviewed Journal) - (15-Dec-03)

IMMUNIZATION OF MICE WITH PLASMID DNA CODING FOR NCGRA7 OR NCSHSP33 CONFERS PARTIAL PROTECTION AGAINST VERTICAL TRANSMISSION OF NEOSPORA CANINUM - (Peer Reviewed Journal)

Liddell, S., Parker, C.C., Vinyard, B.T., Jenkins, M.C., Dubey, J.P. 2003. Immunization of mice with plasmid dna coding for ncgra7 or ncshsp33 confers partial protection against vertical transmission of neospora caninum. Journal of Parasitology. 89:496-500.

IMMUNOLOGICAL AND MOLECULAR STRATEGIES TO CONTROL NEOSPOROSIS IN CATTLE - (Abstract)

Tuo, W., Dubey, J.P., Gasbarre, L.C., Jenkins, M.C., Zarlenga, D.S., Fetterer, R.H., Dyer, R., Boyd, P., Paape, M.J. 2003. Immunological and molecular strategies to control neosporosis in cattle. BARC Poster Day.

NEOSPORA VACCINATION IN AN OVINE MODEL FOR PREVENTION OF CONGENITAL TRANSMISSION - (Peer Reviewed Journal)

O'Handley, R.M., Morgan, S.A., Parker, C.C., Jenkins, M.C., Dubey, J.P. 2003. Neospora vaccination in an ovine model for prevention of congenital transmission. American Journal of Veterinary Research 64:449-452.

ADVANCING CRYPTOSPORIDIUM PARVUM DETECTION METHODOLOGIES - (Book/Chapter)

JENKINS, M.C., TROUT, J.M., HIGGINS, J.A., SMITH, J., VEAL, D., DORSCH, M. ADVANCING CRYPTOSPORIDIUM PARVUM DETECTION METHODOLOGIES. American Waterworks Association Research Foundation. ISBN 1-58321-266-3. 2002.

OCCURRENCE OF NEOSPORA CANINUM ANTIBODIES IN SERA FROM DOGS OF THE CITY OF SAO PAULO, BRAZIL - (Peer Reviewed Journal)

Gennari, S.M., Yai, L.O., D'Auria, S.R., Cardoso, S.S., Kwok, O.C., Jenkins, M.C., Dubey, J.P. 2002. Occurrence of neospora caninum antibodies in sera from dogs of the city of sao paulo, brazil. Veterinary Parasitology 106:177-179.

TEMPORAL VARIABILITY OF CRYPTOSPORIDIUM IN THE CHESAPEAKE BAY - (Peer Reviewed Journal)

FAYER, R., TROUT, J.M., JENKINS, M.C., XIAO, L., GRACZYK, T.K. TEMPORAL VARIABILITY OF CRYPTOSPORIDIUM IN THE CHESAPEAKE BAY. PARASITOLOGY RESEARCH. 2002. Vol. 88:998-1003.