Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Mapping

Efforts Toward the Development of a Transcription Map of Human Chromosome 16
The Los Alamos Genomics Group. Presented by Amanda Ford and Cleo Naranjo.

Gene Expression in Hydatidiform Mole
Rebecca Lobb, Cheryl Lemanski, Karen Denison, and Joe Gatewood

Fluorescent in situ Hybridization Mapping of cDNA Clones from an Unusual Human Library
Karen Denison and Joe M. Gatewood

Human cDNA Mapping by Hybridization to High-density megaYAC Dot Blots
Lucy Ling, Betty Borsody, JoAnn Dubois, Jonathan Norcross, Kathy Falls, Romina Bashirzadeh, Liam Haveran, Teresa Kanjuparamban, Ron Lundstrom, and Donald T. Moir

Gene Isolation From Human Chromosome 19
Greg Lennon, Dominique Giorgi, Kimberly Lieuallen, Len Pennacchio, Christa Prange, and Sylvie Rouquier

Mouse-RNAs for the Determination of Stage- and Tissue-Specific Expression Profiles for Human Genes Throughout Development
Dabney K. Johnson and Lisa J. Stubbs

Integrated physical mapping of human cDNAs
Mihael H. Polymeropoulos

Utilization of normalized libraries in cDNA selection experiments to identify transcribed sequences in genomic DNA
Marcelo Bento Soares, Maria de Fatima Bonaldo, Pierre Jelenc, Lee Lawton, Long Su & Argiris Efstratiadis

Application of Fluorescence in situ Hybridization in Genome Analysis
Barbara J. Trask, Hiroki Yokota, Cynthia Friedman, Hillary Massa, Eric Green, Jim Evans, Antonia Martin-Gallardo, Janey Youngblom, and Ger van den Engh

Production of probes at the Resource for Molecular Cytogenetics
W.-L. Kuo, C. Collins, L. Daneshvar, K. Greulich, D. Kowbel, J. Marstaller, D. Pinkel, L. Riedell, F. Shadravan, M. Wang, U. Weier, P. Yue, M. Zorn, and J. Gray

Reagents For Understanding And Sequencing The Human Genome
J. R. Korenberg, X.N. Chen, S. Gerwehr and
BAC: S. Mitchell, R. Hubert, U.-J. Kim, H. Shizuya, M. Simon, K. Yamakawa.
cDNA: M. Adams, K. Becker, K. Denison, P. Drew, J. Gatewood, G. Guellaen, L. Hood, D. Hwang, G. M. Huang, C.-C. Liew, J. C. Venter

Strand-Specific In Situ Hybridization: Application of the Method
Julianne Meyne, Edwin H. Goodwin, Susan M. Bailey, Loanne R. Smith, Denise I. Quigley and Robert K. Moyzis

Time-Gated Flow Analysis of Human Chromosomes
V.V. Zenin, N.D. Akselov, A.N. Shatrova, Y. V. Kravatskj, A. Kuznetsova, L. S. Cram, A. I. Poletaev

Hybridization Technology Development at the Resource for Molecular Cytogenetics
H.-U. Weier, C. Thompson, M. Wang, K. Greulich, C. Collins, J. Gray and D. Pinkel

CGH Imaging for Routine Use
Damir Sudar, Jim Mullikin, Steve Lockett, Jim Piper, Gus van der Peltz, David Kaszuba, Mark de Kanter, Dan Pinkel, Joe Gray

High Resolution Mapping by Scanning Probe Microscopy
D. P. Allison, T. Thundat, R. J. Warmack, Colin Collins and Joe Gray

Construction And Characterization of Region-Specific Microdissection Libraries for Human Chromosome 2
Fa-Ten Kao and Jingwei Yu

Human Artificial Episomal Chromosomes (HAECs) for Cloning, Mapping and Functional Testing of Large Genetic Units in Human Cells
Tian-Qiang Sun, Michael Grosz, Zachary Kelleher & Jean-Michel H. Vos

A Human DNA Library in Bacterial Artificial Chromosomes (BACs): Application to Physical Mapping of Chromosome 22
Hiroaki Shizuya, Ung-Jin Kim, Bruce Birren, Tania Slepak, Valeria Mancino, April Mengos and Melvin Simon

Bacterial Artificial Chromosomes (BACs) for Mapping and Sequencing
Melvin I. Simon, Ung-Jin Kim, Hiroaki Shizuya, Bruce Birren, and Jerry Solomon

Generation and Characterization of a Large Insert Chromosome 2 PAC Library
Denise Boehrer and Jeffrey C. Gingrich

Construction of improved vectors for the preparation of PAC libraries
Eirik Frengen, Panayotis A. Ioannou, Chira Chen, Eugenia Pietrzak, Xiaoping Guan, Joe Capehart, Joel Jessee, Hans Lehrach and Pieter J. de Jong

A Panel Of Mouse/Human Monochromosomal Hybrid Cell Lines Each Containing A Single Different Tagged Human Chromosome
Arbansjit K. Sandhu, G. Pal Kaur and Raghbir S. Athwal

Construction of a 2 Mb YAC library in transgenic mice of human chromosome 21q22.2
Desmond J.Smith, Yiwen Zhu, Jan-Fang Cheng, Edward M. Rubin

Construction of DNA Libraries From Flow Sorted Human Chromosomes
Larry L. Deaven, Mary K. McCormick, Deborah L. Grady, Donna L. Robinson, Judy M. Buckingham, Nancy C. Brown, Evelyn W. Campbell, Mary L. Campbell, John J. Fawcett, Phil Jewett, Jonathan L. Longmire, Adelmo Martinez, Linda J. Meincke, Pat L. Schor, and Robert K. Moyzis,

Chromosome Specific Cosmid and Lambda Libraries from Flow-Sorted Chromosome
Jeffrey A. Garnes, Benjamin S. Wong, Wanda Johnson, Jerry Eveleth, Anne Bergmann, Richard Langlois, Anthony V. Carrano, and Jeffrey C. Gingrich

Efficient Pooling of YAC Libraries
David J. Balding, David C. Bruce, William J. Bruno, Norman A. Doggett, Emanuel Knill, David C. Torney, and Clive C. Whittaker

Generation of Transgenic Mice Carrying Centromeric Sequences
Ana V. Perez-Castro, Julie Wilson, Michael Altherr and Robert K. Moyzis

Comparative Mapping of a Conserved Zinc-Finger Gene Cluster in Man and Mouse
Mark Shannon, Michael L. Mucenski, Linda Ashworth, and Lisa Stubbs

Detailed Man-Mouse Comparative Maps of Human Chromosome 19
Johannah Doyle, Estela Generoso, William Dunn, Beverly Stanford, Ethan Carver, Eugene Rinchik, Susan Watt, Wolfgang Zimmermann, Linda Ashworth, Greg Lennon, Anne Olsen, Susan Tsujimoto, Harvey Mohrenweiser, Brigitte Brandriff and Lisa Stubbs

Development and Testing of a Method for the Purification of Evolutionarily Conserved Sequences from Cloned Human DNA
Lisa Stubbs, Karen Glantz, and Elbert Branscomb

Functional Analysis of Genes Using the Mouse as a Model System
Peter R. Hoyt, Theresa A. Banks, Christopher Bartholomew, Patrick J. Blair, Virginia L. Godfrey, Marilyn K. Kerley, William H. Lee, Brigid L. M. Hogan, Barry T. Rouse, James N. Ihle, S.Steven Potter, and Michael L. Mucenski

Mouse Genome Studies Aimed at Deciphering Gene Function
R.P. Woychik, L. Stubbs, M.L. Mucenski, J. Moyer, H. Kwon, W.G. Richards, B. Yoder, and J.E. Wilkinson

Sequence-Directed DNA Manipulation for Enhanced Mapping and Sequencing
Charles R. Cantor, Natalia Broude, Takeshi Sano, Kazuhiro Tsukamoto, Marek Przetakiewicz, A. Burak. Dalan, Abha Chandra, Rhonda Harrison, Nikolay Bukanov, Ronald Yaar, Demetri Moustakas, Przemekyslaw Szafranski, and Cassandra L. Smith

Marker development for the EPM1 region of human chromosome 21, q22.3
J.A. Warrington, K. O'Connor, S. Hebert, M. Harris, R. Goold, A.E. Lehesjoki, A. de la Chapelle, N. Stone, R. Myers and D.R. Cox

Current status of the integrated physical map of chromosome 16
D.F. Callen, S. Apostolou, S. Lane, S.A. Whitmore, N.A. Doggett, R.I. Richards, J.C. Mulley, G.R. Sutherland

Progress with a strategy for cosmid closure of band 16q24.3
D.F. Callen, S. Apostolou, S. Lane, S.A. Whitmore, N.A. Doggett, R.I. Richards, J.C. Mulley, G.R. Sutherland

Mapping Sequence Tagged Sites to Distinct Segments of Chromosome 16
The Los Alamos Genomics Group and D.F. Callen. Presented by M.R Altherr

An Integrated Map of Human Chromosome 16
N.A. Doggett, D.F. Callen, M.R. Altherr, L.A. Duesing, J.G.Tesmer, L.J. Meincke, D.C. Bruce, A.A. Ford, D.C. Torney, R.D. Sutherland, M.G. Lowenstein, M.O. Mundt, W.J. Bruno, E.H., Knill, R.I. Richards, G.R. Sutherland, L.L. Deaven, and R.K. Moyzis

Analysis of External YAC Data for Incorporation into the Chromosome 16 Map
R.D. Sutherland, R.M. Pecherer, L.A. Duesing, and N.A. Doggett

Construction of an STS-Content YAC Map of Human Chromosome 16
N.A. Doggett, L.A. Duesing, J.G.Tesmer, L.J. Meincke, M.R. Altherr, A.A. Ford, D.C. Bruce, D.C. Torney, E.H., Knill, W.J. Bruno, R.D. Sutherland, M.G. Lowenstein, M.O. Mundt, D.F. Callen2, G.R. Sutherland2, L.L. Deaven, and R.K. Moyzis

Development of a Multi Level Resolution Map of the p arm of Chromosome 19 by Integration of FISH, YAC, Cosmid Contig, and Restriction Map Data
Susan A. Allen, Anca M. Georgescu, Maria T. de Jesus, Susan M. G. Hoffman, Gregory G. Lennon, Anthony V. Carrano and Anne S. Olsen

A Metric Physical Map of Human Chromosome 19
Linda K. Ashworth, (ashworthl@llnl.gov), Joe Balch, Mark Batzer, Brigitte Brandriff, Elbert Branscomb, Emilio Garcia, Jeff Garnes, Jeff Gingrich, Jane Lamerdin, Richard Langlois, Greg Lennon, Ray Mariella, Harvey Mohrenweiser, Anne Olsen, Tom Slezak, and Anthony V. Carrano

Human Chromosome 19: A Fluorescence in situ Hybridization Map with Genomic Distance Estimates for 196 Sequential Intervals Separating 198 Ordered Cosmid Reference Points and Spanning 50 Mb
Brigitte F. Brandriff, Laurie A. Gordon, Anne S. Olsen, Anthony V. Carrano, Anne Bergmann, Mari Christensen, Anne Fertitta, Linda Danganan, Denise Lee, Linda K. Ashworth, David O. Nelson, and Harvey W. Mohrenweiser

Chromosome 19 closure: High Resolution Physical Map of the Entire q12 and q13.1 Bands
Jeffrey Elliott, Ann Gorvad, K. Soliman, Brian Cheney, Linda K. Ashworth, Matt Burgin, Jane S. Lamerdin, Greg Lennon, Anthony V. Carrano, and Emilio Garcia

Chromosome 19 Closure: High Resolution Physical Map of an Eight Megabase Region Extending from q12 to q13.1
Emilio Garcia, Jeff M. Elliott, Ann Gorvad, Linda K. Ashworth, Matt Burgin, Greg Lennon, Jane S. Lamerdin and Anthony V. Carrano

Incorporation of FISH Reference Clones, Genetic Markers and Genes into the Physical Map of Human Chromosome 19
Susan Tsujimoto, Annette Swartz, Laurie Gordon, Anthony V. Carrano, Brigitte Brandriff, and Harvey W. Mohrenweiser

EcoRI Restriction Mapping of Chromosome 19
Matt Burgin, Linda K. Ashworth, Stephanie Johnson, Laurie Gordon, Susan Hoffman, Anthony V. Carrano

Distribution and Organization of Zinc Finger Genes on Chromosome 19
Susan M. G. Hoffman, Chris Amemiya, and Harvey W. Mohrenweiser

Cosmid Contig Map of a 900-kb Candidate Region for Finnish Congenital Nephrosis in 19q13.1
Anne Olsen, Anca Georgescu, Laurie Gordon, Matt Burgin, Marjo Kestila, Minna Mannikka, and Karl Tryggvason

Generation and High Resolution Mapping of New STSs Derived From Chromosome 2 Microdissection Clones
Jeffrey C. Gingrich, Jeffrey A. Garnes, Jane E. Lamerdin and Lisa K.Scheidecker

Analysis of Randomly Cloned Chromosome 5 DNA Sequences for Similarities to GenBank Sequences
Robert Wagner, Deborah Grady, Julie Houts, Donna Robinson, Joe Gatewood, Sue Thompson, Larry Deaven, and Robert Moyzis

Low Resolution Physical Mapping of Human Chromosome 5: Cloning the Cri-du-Chat Critical Regions
D.L. Grady, D.L. Robinson, J. Overhauser, S. Goodart, M. Feder, A. Simmons, M. Lovett, E. Nickerson, J.D. McPherson, J.J. Wasmuth, E.T. Peterson, L.A. Chasteen, L.L. Deaven, and R.K. Moyzis

Construction of a P1 Map in the Region of Chromosome 5q31-q35
Jan-Fang Cheng, Steve Lowry, Yiwen Zhu, Duncan Scott and Eddy Rubin

Construction of a High Resolution P1 and cDNA Map in the Down Syndrome Region of Chromosome 21
Jan-Fang Cheng and Yiwen Zhu

Long range mapping and sequencing of the human X chromosome
D.L. Nelson, E.E. Eichler, B.A. Firulli, Y. Gu, G.B. Ferraro, B. Franco, A. Grillo, G. Borsani, M.C. Wapenaar, A. Ballabio, A.C. Chinault, E.J. Roth, H.Y. Zoghbi, F. Lu, M.A. Wentland, D.M. Muzny, J. Lu, R.L. Clingan, S. Richards, R.A. Gibbs

Cosmid Contig Construction and Gene Identification in Human Xq28
J.E. Parrish, E.E. Eichler, Y. Gu, B.A. Oostra, A.J.M.H. Verkerk, J. Reynolds, C.S. Richards, A.S. Spikes, L.G. Shaffer and D.L. Nelson

Towards an STS content map of human chromosome 11: Localization of 910 YAC clones and contigs and assembly of a first generation physical map
Glen A. Evans, Julie M. Bailis, Sylvia Thomas, Jason V. Khristich, Karin Diggle, Yelena Marchuck, Joshua Tobin, Stephen P. Clark, Annie Rodkins, Stewart Marcano, Allan C. Churukian, Jane S. Hutchinson, Yalin H. Wei, Ron Scott, Kim Jackson, Lori Romberg, Shane Probst, David Burbee, Michael W. Smith, Licia Selleri, John Quackenbush and Harold R. Garner

Building a Physical Map by STS Content Mapping to Support Large-Scale Genomic Sequencing: The Drosophila Genome Project
William J. Kimmerly, Karen E. Stultz, Keith Lewis, Veronica M. Lustre, Dazhong Sun, Christopher H. Martin, and Michael J. Palazzolo

Generating and Typing CAPS Genetic Markers in Dog
Mark W. Neff, Mike Strathmann, and Jasper Rine

Physical Mapping in Preparation for DNA Sequencing
Andreas Gnirke, Regina Lim, Gane Wong, Jun Yu, and Maynard Olson

Identification and Molecular Cloning of a 20q13.2 Amplicon in Breast Carcinoma
Colin Collins, Jeff Froula, Minna Tanner, Jan-Fang Cheng, David Kowbel, Farideh Shadravan, Chris Martin, Michael Palazollo, Mary Hintz, Ulli Weier, Wen-Lin Kuo, Olli Kallioniemi, Jeff Gingrich, Johanna Rommens, Dan Pinkel and Joe Gray

Cloning of a Human Damage-specific DNA Binding Protein
Anne F. Nichols, Tom Brody, Scott Keeney, and Stuart Linn

The role of recombination and RAD52 in mutation of chromosomal size DNA transformed into yeast
Vladimir Larionov, Joan Graves, Natalya Koupnna, and Michael Resnick

Ligase chain reaction using colorimetric detection
Layne Huiet, Annette Tumolo, Luis Ugozzoli, Tony Reyes, Jimmie Lowry, Bruce Wallace and Frank Witney

Resource for Molecular Cytogenetics
C. Collins, L. Daneshvar, Karin Greulich, D. Kowbel, W.-L. Kuo, L Riedell, F. Shadravan, D. Sudar, J. Mullikin, S. Lockett, P. Yue, U. Weier, Manfred Zorn, D. Pinkel, J. Gray

Noncanonical Octanucleotide Sequences Recognized with the POU Domain of Oct-2B Factor
Alexander G. Stepchenko, N.N.Luchina and Oleg L. Polanovsky

Chemiluminescent Detection of Multiplex Labeled Microsatellite Markers and DNA Sequences
Chris S. Martin, Laurie Butler, Greg Schneider and Irena Bronstein

Interaction of Dimeric Intercalating Fluorescent Dyes with Single-Stranded DNA
Hays S. Rye and Alexander N. Glazer

Photo-Anchoring Probes for In Situ Localization
A. I. Poletaev, T. V. Nasedkina, T. S. Godovikova and D. G. Knorre

Comparative Analysis of Human DNA Variations by Fluorescence-based Sequencing of PCR Products
Pui-yan Kwok, Christopher Carlson, Thomas D. Yager, Wendy Ankener, and Deborah Nickerson

Alu Repeats: Source for the Genesis of Simple Sequence Repeats
Santosh S. Arcot, Hernan Bazan, Prescott L. Deininger and Mark A. Batzer

Alu Repeats: Tools for Genomics and Modes of Evolution
Mark A. Batzer, David H. Kass, Michelle Alegria-Hartman, and Prescott L. Deininger

YACs with Unique Capture Handles
Lucy L. Ling, Jonathan Norcross, Elizabeth Borsody and Donald T. Moir

Mapping Markers in Genomic Library Clones Using Oligonucleotide Arrays
R. Lipshutz, R.J. Sapolsky, S.P.A. Fodor

Transformation-associated recombination in yeast can be used to generate large fragment (YAC) genome libraries
V. Larionov, N. Kouprina, J. Graves, and M.A. Resnick