Marion D. Johnson III and Jacques R. Fresco 
Princeton University, Department of Molecular Biology, Princeton, New Jersey 
mjohnson@molbio.princeton.edu, jrfresco@princeton.edu 

55. Optical Mapping: A Complete System For Whole Genome Shotgun Mapping 

D.C. Schwartz^1,2, T. Anantharaman², J. Apodaca¹, C. Aston¹, V. Clarke¹, D. Gebauer¹, S. Delobette¹, E. Dimalanta¹, J. Edington¹, A. Evenzehav¹, J. Giacalone¹, V. Gibaja¹, C. Hiort¹, E. Huff¹, J. Jing¹, Z. Lai¹, D. Lazaro¹, E. Lee¹, J. Lin¹, K. Lin¹, B. Mishra², L. Ni¹, S. Paxia², B. Porter¹, R. Qi¹, A. Ramanathan¹, Y. Skiadis¹, J. Vafai¹, W. Wang¹, H. Zhao^1 
1W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, and ²Courant Institute of Mathematical Sciences, Department of Computer Science, New York University, NY 10003 
schwad01@mcrcr6.med.nyu.edu 

56. Verifying Sequence By Atomic Force Microscopy 

David P. Allison and Peter R. Hoyt 
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6123 
allisondp@ornl.gov 

57. Molecular Cytogenetics Comes of Age: A Resource that Extends From "T" to Shining "T"  

J. R. Korenberg¹, X. N. Chen¹, D. Noya¹, X.Wu², B. Birren², T. Hudson^2,3 
1 Medical Genetics Birth Defect Center, Cedars-Sinai Medical Center, University of California at Los Angeles, Los Angeles, California; ²Whitehead Institute, Massachusetts Institute of Technology, Massachusetts; and ³McGill University, Montreal, Canada 
jkorenberg@xchg.peds.csmc.edu 

58. Automated Purification of Blood, or Bacterial Genomic DNA 

Dan P. Langhoff, Tuyen Nguyen, and William P. MacConnell 
MacConnell Research Corporation, San Diego, California 
macres@macconnell.com 

59. New Host Strains for Stabilization and Modification of YAC Clones 

Natalay Kouprina, Maxim Koriabine, and Vladimir Larionov 
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, P.O. Box 12233, Research Triangle Park, NC 27709 
larionov@niehs.nih.gov 

60. Direct Isolation of a Centromeric Region from a Human Mini-Chromosome by in Vivo Recombination in Yeast 

Natalay Kouprina, Motonobu Katoh, Mitsuo Oshimura, and Vladimir Larionov 
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC 27709 
kouprina@niehs.nih.gov 

61. Insert Clone Selection by Sorting GFP-Expressing E. coli 

Juno Choe and Ger van den Engh 
Department of Molecular Biotechnology, University of Washington, WA 98195 
choe@biotech.washington.edu 

62. A Resource of Mapped BAC Clones for Identifying Cancer Chromosome Aberrations 

Norma J. Nowak¹, Jeffrey Conroy¹, Greg P. Caldwell¹, Joseph Catanese¹, Barbara Trask², John D. McPherson³, David R. Bentley⁴, Grace Shen⁵, and Pieter J. de Jong^1 
1Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263; ²Department of Molecular Biotechnology, University of Washington School of Medicine, Seattle, WA 98195; ³Department of Genetics, Washington University School of Medicine, St. Louis, MO 63108; ⁴Sanger Centre, Hinxton, Cambridge, CB10-1RQ, UK and 5CCAP Program; and ⁵National Cancer Institute, Bethesda, MD 
nowak@dejong.med.buffalo.edu 

63. Preparation of New BAC Vectors for BAC Cloning and Transformation- Associated Recombination ("TAR") Cloning 

Changjiang Zeng¹, Yu Wang¹, Kazutoyo Osoegawa¹, Natasha Kouprina², Vladimir Larionov², and Pieter J. de Jong^1 
1Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263 and ²Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC 27709 
zeng@dejong.med.buffalo.edu 

64. "RPCI" Human  and Mouse Bacterial Artificial Chromosome Libraries: Construction and Characterization  

Kazutoyo Osoegawa¹, ChungLi Shu¹, Baohui Zhao¹, Minako Tateno², Eirik Frengen¹, Joseph J. Catanese¹, Yoshihide Hayashizaki² and Pieter J. de Jong^1 
1Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263 and ²Genome Science Laboratory, Riken Tsukuba Life Science Center, Japan 

65. Characterization of a BAC Clone Resource for Human Genomic Sequencing: Analysis of 150 Mb of Human STCs and Implications for Human Genomic Sequencing 

G. G. Mahairas, J. C. Wallace, J. Furlong, K Smith, S. Swartzell, A. Keller, HTSC Staff and L. Hood 
High Throughput Sequencing Center, University of Washington, Seattle, WA 98109 
gmahaira@u.washington.edu 

66. Human BAC End Sequencing 

Shaying Zhao, Mark Adams, Bill Nierman, and Joel Malek 
TIGR, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville MD 20850 
szhao@tigr.org 

67. Construction of a Genome-Wide Human BAC-Unigene Resource 

Bum-chan Park¹, Robert Xuequn Xu, Chang-Su Lim, Mei Wang, Aaron Rosin, Steve Mitchell, Hee Moon Park¹, Eunpyo Moon², Ung-Jin Kim, and Melvin I. Simon 
Division of Biology, Caltech, Pasadena, CA 91125 
¹Chungnam University, Taejon, Korea and ²Ajou University, Suwon, Korea 
simonm@cco.caltech.edu 

68. A New Bacterial Artificial Chromosome (BAC) Vector, a Large-Insert (Average of Over 200 kb) BAC Library of the Human, and an Improved Method of Construction of BAC Libraries 

Sangdun Choi, Yu-Jiun Chen, Mel Simon, and Hiroaki Shizuya 
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA 
schoi@cco.caltech.edu 

69. One Tier Pooling of a Total Genomic BAC Library 

D.C. Torney, J.L. Longmire, D.C. Bruce, J. Fawcett, M. Campbell, J. Tesmer, M. Maltbie, B. Taggett, T. Tatum, P. Jewett, J. Meyne, N. Lenhert, Y. Valdez, S. Bailey, A. Schliep¹, L.L. Deaven, and N.A. Doggett 
Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545 and ¹University of Cologne, Cologne, Germany 
doggett@gnome.lanl.gov 

70. High Density Colony Filter Production and Automated Data Analysis for Efficient Hybridization Screening of BAC Libraries 

Anca Georgescu, Laura Kegelmeyer, Bernadette Lato, Hummy Badri, Matthew Groza, and Anne Olsen 
Human Genome Center, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551 
olsen2@llnl.gov 

71. Systematic Conversion of a YAC/STS Map into a Sequence Ready BAC Map 

C. Han and N.A. Doggett 
Joint Genome Institute, Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545 
chan@telomere.lanl.gov 

72. An Arrayed BAC Resource for the High Resolution Mapping of Cancer-Related Chromosome Aberrations 

Eunpyo Moon¹, Jonghyeob Lee¹, Mei Wang, Bum-Chan Park, Ken Myambo², Colin Collins², Melvin Simon, Ung-Jin Kim 
¹Ajou University, Suwon, Korea 
² Lawrence Berkeley National Laboratory, Berkeley, California 
simonm@cco.caltech.edu 

73. A 12 Mbp Completely Contiguous Sequence-Ready BAC Contig in Human Chromosome 16p13.1-11.2 

Yicheng Cao, Hyung Lyun Kang, So Hee Dho¹, Diana Bocskai, Mei Wang, Xuequn Xu, Jun-Ryul Huh¹, Byeong-Jae Lee¹, Francis Kalush², Judith G. Tesmer³, Eunpyo Moon⁴, Norman A. Doggett³, Mark D. Adams², Melvin Simon, and Ung-Jin Kim 
¹ Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea; ² The Institute for Genomic Research, Rockville, Maryland; ³ Los Alamos National Laboratory, Los Alamos, New Mexico; and ⁴ Ajou University, Suwon, Korea 
simonm@cco.caltech.edu 

74. Completing the Sequence-Ready Map of Chromosome 19 

Laurie Gordon, Anca Georgescu, Mari Christensen, Sha Hammond, Hummy Badri, Bernadette Lato, Matthew Groza, Linda Ashworth, Mark Wagner, and Anne Olsen 
Human Genome Center, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551 
olsen2@llnl.gov 

75. High-Throughput Multiplexed Fluorescent-Labeled Fingerprinting of BAC Clones 

Yan Ding¹, Martin D. Johnson², Wang Q. Chen³, Gigi E Park¹, Yujin Chen¹, and Hiroaki Shizuya^1 
1Beckman Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, U. S. A; ²PE Biosystems, 850 Lincoln Center Drive, Foster City, CA 94404, U. S. A.; and ³Paracel Inc., 80 S. Lake Ave #650, Pasadena, CA 91101-2616, U. S. A. 
yding@cco.caltech.edu 

76. Progress Towards a High Resolution Sequence-Ready Map of Human Chromosome 5 

Steve Lowry, Ze Peng, Duncan Scott, Yiwen Zhu, Mei Wang, Roya Hosseini, Michele Bakis, Joel Martin, Ingrid Plajzer-Frick, Jeff Shreve, and Jan-Fang Cheng 
Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 
jcheng@mhgc.lbl.gov 

77. High Throughput Fingerprinting and Contig Assembly to Supply Sequence Ready Templates to the JGI-PSF 

Linda Meincke, Robert Sutherland, Connie Campbell, Joe Fawcett, Phil Jewett, Lynn Clark, Cliff Han, Larry Deaven, and Norman Doggett 
Joint Genome Institute, Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545 
meincke@telomere.lanl.gov