U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R87
Trypsin-EDTA Solution, lX

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Trypsin (1:250)* 0.05 g
EDTA, disodium salt 0.02 g
NaCl, 0.9% 100 ml

 

Dissolve trypsin and EDTA in the NaCl diluent. Filter-sterilize through 0.22 µm membrane.


*1 g Trypsin (1:250) will digest 250 g casein substrate under standard conditions.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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