U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R77
Standard Saline Citrate (SSC) Solution (20%)

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NaCl (reagent grade) 175.3 g
Sodium citrate 88.2 g

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.


6X SSC


NaCl (reagent grade) 52.6 g
Sodium citrate 26.5 g

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.



3X SSC


6X SSC 500 ml
Deionized water 500 ml


2X SSC


6X SSC 333 ml
Deionized water 667 ml




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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