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R89
Voges-Proskauer (VP) Test Reagents
-Naphthol | 5 g |
Alcohol (absolute) | 100 ml |
Potassium hydroxide | 40 g |
Distilled water to make 100 ml |
Voges-Proskauer (VP) test
Transfer 1 ml of 48 h culture to test tube and add 0.6 ml solution 1 and 0.2 ml solution 2. Shake after adding each solution. To intensify and speed reaction, add a few creatine crystals to mixture. Let stand at room temperature. Read results 4 h after adding reagents.
Development of eosin pink color is positive test.
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