Project Number: 5325-21000-015-00
Project Type: Appropriated

Start Date: May 15, 2008
End Date: May 14, 2013

Objective:
1) Apply molecular biology and genomics to understand the structure, function and expression of wheat seed protein genes; 2) Contribute to the understanding of the organization of the wheat genome, with a focus on the group 1 chromosomes; and 3) Develop and utilize Brachypodium as a model system for Triticeae research for grain quality and other traits.

Approach:
1) Use combinations of molecular alterations, heterologous expression, and plant transformations in the application of molecular biology and genetic engineering to wheat high-molecular-weight (HMW) glutenin class seed storage protein genes and other seed protein classes to understand more of the molecular bases of wheat quality and utilization. 2) Contribute to the understanding of the organization of the wheat genome through in-depth studies of wheat seed protein genetic loci and participation in national and international wheat genome characterization projects. 3) Employ DNA microarrays to profile gene expression during wheat seed development and changes in storage protein gene activity and wheat quality. 4) Contribute to the development of Brachypodium as a model for small grains research, to include use in studying wheat seed protein gene controls. BSL-1 (4/08). Previously was 5325-21430-011-00D (10/03). Replacing 5325-21000-011-00D (04/08).