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Research Project:
CONTROL OF FUSARIUM MYCOTOXINS IN CORN, WHEAT, AND BARLEY
Location: Mycotoxin Research
Title: Natural product chemistry meets genetics: When is a genotype a chemotype?
Author
Submitted to: Journal of Agricultural and Food Chemistry
Publication Type:
Review Article
Publication Acceptance Date: July 10, 2008
Publication Date: September 10, 2008
Citation: Desjardins, A.E. 2008. Natural product chemistry meets genetics: When is a genotype a chemotype? Journal of Agricultural and Food Chemistry. 56(17):7587-7592.
. Journal of Agricultural and Food Chemistry.
Interpretive Summary: This review discusses applications of DNA testing to predicting natural product chemotypes of fungi and plants of importance in food and agriculture. This review provides standards and guidelines for scientists and the food safety industry in use and interpretation of DNA testing for prediction of mycotoxins in foods and feeds.
Technical Abstract: The chemotype of a microbial or plant species has traditionally been defined as its profile of natural products, and the genotype as its genetic constitution or DNA sequence. The purpose of this perspective is to discuss applications of DNA genotyping, particularly by PCR-amplification methods, to predicting natural product chemotypes of fungi and plants of importance in food and agriculture. Development of PCR-genotyping for predicting chemotypes will require collaboration between molecular biologists and natural product chemists, as well as community standards for reporting data. PCR-genotyping should be validated by chemical analysis of individuals that represent the allelic diversity of the target gene in the population. To avoid misinterpretation, it is critical to differentiate data obtained by genotyping from data obtained by chemical analysis. The obvious and appropriate solution is to retain the established meanings of genotype and chemotype, both of which have been in use for half a century in the fields of genetics and natural product chemistry.
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Last Modified: 10/23/2008
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