2007 Annual Report 1a.Objectives (from AD-416) The objective this cooperative research project is to develop detection and monitoring methods, and Attract-and-Kill and control strategies, for the Asian Longhorned Beetle, ANOPLOPHORA GLABRIPENNIS (ALB). More specifically, we will determine the relative attraction of ALB to sentinel trees and artificial lures; the efficacy of an encapsulated insecticide when used in combination with sentinel trees and when applied to landscape trees; and the indigenous geographic and host range of ALB in China. 1b.Approach (from AD-416) Field studies will be conducted. (1) Adult ALB attraction to potted Acer mono, Acer truncatum and Acer platanoides trees will be evaluated, and relative seasonal abundance of adult ALB among the adjoining infested willow trees measured, under natural field conditions in Tianjin and/or other appropriate field sites. (2) Adult ALB attraction to potted Acer mono trees, half of which have and half of which have not been treated with an encapsulated insecticide, will be evaluated, and relative seasonal abundance of adult ALB among the adjoining infested willow trees measured, under natural field conditions in Tianjin and/or other appropriate field sites. (3) Relative efficacy of an encapsulated insecticide to control adult ALB will be evaluated when applied at different rates to ALB-infested Acer negundo landscape trees in Yanji. (4) Adult ALB attraction to different host odor blends will be evaluated, and relative seasonal abundance of adult ALB among the adjoining infested willow trees measured, under natural field conditions in Tianjin and/or other appropriate field sites, including evaluation of formulation, blend ratio and attractive radius. (5) ALB specimens (larvae and adult) will be collected across the indigenous geographic and host range of ALB in eastern China and specimens will be evaluated in the U.S. to identify the indigenous geographic and host range of ALB. This research will collectively complement of our ongoing investigations and result in tools for prediction, detection, monitoring, eradication and management of ALB. 3.Progress Report This report serves to document research conducted under a specific cooperative agreement between ARS and the Chinese Academy of Agricultural Sciences Beijing, CHINA). Additional details of research can be found in the report for 1926 22000 019 00D, Development of Biological Control and Related Technologies for Invasive Insect Pests with Emphasis on the Asian Longhorn Beetle. The objective of this cooperative research project is to develop detection and monitoring methods, and Attract-and-Kill and control strategies, for the Asian Longhorned Beetle (ALB), ANOPLOPHORA GLABRIPENNIS. More specifically, (1) to determine the relative attraction of sentinel trees and artificial lures for detection of ALB; (2) to determine the efficacy of an encapsulated insecticide when used in combination with sentinel trees (detection) and when applied to landscape trees (population suppression); (3) to determine the indigenous geographic and host range of ALB in China; and (4) to identify potential natural enemies for introduction to the U.S. Progress Report: Field studies were conducted in China, including development of sentinel trees for detection of ALB and insecticidal treatments for control of this pest. Results from the following studies are strongly influenced by environmental factors and ALB population levels. Therefore, these studies must be conducted over multiple years to insure repeatability and reliability of results. Host Selection and Host Preference: Sentinel Trees (detection of adult ALB). In FY07, the second year of a three year study in Tianjin, we continued to evaluate the attraction of adult ALB to potted ACER MONO trees, half of which have and half of which have not been treated with an encapsulated insecticide. Integral to these studies, the relative seasonal abundance of adult ALB among the adjoining infested willow trees was also monitored. Unfortunately, local conditions beyond our control adversely impacted the experiments. Therefore, studies will be repeated in 2008. Population suppression and eradication technology: Contact insecticide. In FY07, the third year of a four year study, we continued to evaluate the relative efficacy of an encapsulated insecticide to control adult ALB in Yanji, Jilin. The insecticide had been applied at a range of rates to ALB-infested ACER NEGUNDO landscape trees in 2005 and 2006. In November 2006 we quantified the effects of the treatments on subsequent population development and successful emergence. Data analysis was completed at BIIRU. These studies were monitored by participation of the ADODR in conducting these studies in China, by conference calls and by regular email communications.