Projects

PREVENTION AND CONTROL OF SHIGA-TOXIGENIC ESCHERICHIA COLI IN LIVESTOCK Appropriated (D)   Accession Number: 410408

CONTROL OF PATHOGENIC AND SPOILAGE BACTERIA ON RED MEAT Appropriated (D)   Accession Number: 410528

COLONIZATION CHARACTERISTCS OF BOVINE RECTO-ANAL JUNCTION TISSUE BY ESCHERICHIA COLI O157:H7 Trust (T)   Accession Number: 412001

EFFECTS ON ESCHERICHIA COLI O157:H7 AND SALMONELLA PREVALENCE AND LEVELS FROM FEEDING A DIRECT FED MICROBIAL (DFM) TO FEEDLOT CATTLE Trust (T)   Accession Number: 412073

EFFECT OF DISTILLERS GRAINS ON E. COLI O157:H7 IN FINISHING FEEDLOT CATTLE Trust (T)   Accession Number: 412678

SOURCE ATTRIBUTION OF ANTIBIOTIC RESISTANT SALMONELLA IN CATTLE PROCESSING PLANTS Trust (T)   Accession Number: 412074

HIDE TO CARCASS TRANSFER OF E. COLI O157:H7 AND SALMONELLA IN SMALL SCALE (LESS THAN 1000 HEAD/DAY) BEEF PROCESSING PLANTS Trust (T)   Accession Number: 412681

ENUMERATION OF SALMONELLA THROUGHOUT THE PORK HARVESTING PROCESS Trust (T)   Accession Number: 411921

Publications

The impact of stress level on fecal bacteria and pathogen shedding in feedlot cattle. - (Proceedings/Symposium)

Brown Brandl, T.M., Berry, E.D., Wells, J., Arthur, T.M., Nienaber, J.A. 2008. The impact of stress level on fecal bacteria and pathogen shedding in feedlot cattle. American Society of Agricultural and Biological Engineers. Paper No. 084178. St. Joseph, MI:ASABE.

Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention. Journal of Food Protection. 71(9):1752-1760.

Prevalence and Characterization of Salmonella in Bovine Lymph Nodes Potentially Destined for Use in Ground Beef - (Peer Reviewed Journal)

Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Prevalence and characterization of Salmonella in bovine lymph nodes potentially destined for use in ground beef. Journal of Food Protection 71(8):1685-1688.

Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing - (Peer Reviewed Journal)

Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Guerini, M.N., Wheeler, T.L., Koohmaraie, M. 2008. Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing. Journal of Food Protection 71(3):621-624.

Enumeration of Salmonella from Poultry Carcass Rinses via Direct Plating Methods - (Peer Reviewed Journal)

Harhay, D.M., Arthur, T.M., Koohmaraie, M. 2008. Enumeration of Salmonella from poultry carcass rinses via direct plating methods. Letters in Applied Microbiology 46(2):186-191.

Listeria prevalence and L. monocytogenes serovar diversity at cull cow/bull processing plants in the United States - (Peer Reviewed Journal)

Guerini, M.N., Harhay, D.M., Shackelford, S.D., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2007. Listeria prevalence and Listeria monocytogenes serovar diversity at cull cow and bull processing plants in the United States. Journal of Food Protection. 70(11):2578-2582.

Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America. Journal of Food Protection. 70(7):1622-1626.

Microbiological characterization of lamb carcasses at commercial processing plants in the United States - (Peer Reviewed Journal)

Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Microbiological characterization of lamb carcasses at commercial processing plants in the United States. Journal of Food Protection 70(8):1811-1819.

Enumeration of Salmonella and Escherichia coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Fecal Samples Using Direct Plating Methods - (Peer Reviewed Journal)

Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2007. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. Journal of Applied Microbiology 103(5):1657-1668.

Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter. Journal of Food Protection 70:1076-1079.

Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing. Journal of Food Protection 70(2):280-286.

MICROBIOLOGICAL CHARACTERIZATION OF IMPORTED AND DOMESTIC BONELESS BEEF TRIM USED FOR GROUND BEEF - (Peer Reviewed Journal)

Bosilevac, J.M., Guerini, M.N., Harhay, D.M., Arthur, T.M., Koohmaraie, M. 2007. Microbiological characterization of imported and domestic boneless beef trim used for ground beef. Journal of Food Protection 70(2):440-449.

IMPROVEMENT OF IMMUNOMAGNETIC SEPARATION FOR ESCHERICHIA COLI O157:H7 DETECTION BY THE PICKPEN MAGNETIC PARTICLE SEPARATION DEVICE - (Peer Reviewed Journal)

Nou, X., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2006. Improvement of immunomagnetic separation for Escherichia coli O157:H7 detection by the pickpen magnetic particle separation device. Journal of Food Protection 69:2870-2874.

TREATMENTS USING HOT WATER INSTEAD OF LACTIC ACID REDUCE LEVELS OF AEROBIC BACTERIA AND ENTEROBACTERIACEAE AND REDUCE THE PREVALENCE OF ESCHERICHIA COLI O157:H7 ON PREEVISCERATION BEEF CARCASSES - (Peer Reviewed Journal)

Bosilevac, J.M., Nou, X., Barkocy-Gallagher, G.A., Arthur, T.M., Koohmaraie, M. 2006. Treatments using hot water instead of lactic acid reduce levels of aerobic bacteria and Enterobacteriaceae and reduce the prevalence of Escherichia coli O157:H7 on preevisceration beef carcasses. Journal of Food Protection. 69(8):1808-1813.

EVALUATION OF ESCHERICHIA COLI O157:H7 GROWTH MEDIA FOR USE IN TEST-AND-HOLD PROCEDURES FOR GROUND BEEF PROCESSING - (Peer Reviewed Journal)

Guerini, M.N., Arthur, T.M., Shackelford, S.D., Koohmaraie, M. 2006. Evaluation of Escherichia coli O157:H7 growth media for use in test-and-hold procedures for ground beef processing. Journal of Food Protection. 69(5):1007-1011.

METHODS FOR RECOVERING ESCHERICHIA COLI O157:H7 FROM CATTLE FECAL, HIDE, AND CARCASS SAMPLES: SENSITIVITY AND IMPROVEMENTS - (Peer Reviewed Journal)

Barkocy-Gallagher, G.A., Edwards, K.K., Nou, X., Bosilevac, J.M., Arthur, T.M., Shackelford, S.D., Koohmaraie, M. 2005. Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples: Sensitivity and improvements. Journal of Food Protection. 68:2264-2268.

EVALUATION OF CULTURE- AND PCR-BASED DETECTION METHODS FOR ESCHERICHIA COLI O157:H7 IN INOCULATED GROUND BEEF - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Evaluation of culture- and PCR-based detection methods for Escherichia coli O157:H7 in inoculated ground beef. Journal of Food Protection. 68:1566-1574.

EFFECTS OF LOW-DOSE, LOW-PENETRATION ELECTRON BEAM IRRADIATION OF CHILLED BEEF CARCASS SURFACE CUTS ON ESCHERICHIA COLI O157:H7 AND MEAT QUALITY - (Peer Reviewed Journal)

Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Effects of low-dose, low-penetration electron beam irradiation of chilled beef carcass surface cuts on Escherichia coli O157:H7 and meat quality. Journal of Food Protection. 68(4):666-672.

CHARACTERIZATION OF O157:H7 AND OTHER ESCHERICHIA COLI ISOLATES RECOVERED FROM CATTLE HIDES, FECES, AND CARCASSES - (Research Notes)

Gallagher, G.A., Arthur, T.M., Rivera Betancourt, M., Nou, X., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2004. Characterization of O157:H7 and other Escherichia coli isolates recovered from cattle hides, feces, and carcasses. Journal of Food Protection. 67(5):993-998.

ESCHERICHIA COLI O157 PREVALENCE AND ENUMERATION OF AEROBIC BACTERIA, ENTEROBACTERIACEAE, AND ESCHERICHIA COLI O157 AT VARIOUS STEPS IN COMMERCIAL BEEF PROCESSING PLANTS - (Peer Reviewed Journal)

Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Kent, M.P., Jaroni, D., Pauling, B., Allen, D.M., Koohmaraie, M. 2004. Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriacae, and Escherichia coli O157 at various steps in commercial beef processing plants. Journal of Food Protection. 67(4):658-665.

PREVALENCE OF ESCHERICHIA COLI O157 AND LEVELS OF AEROBIC BACTERIA AND ENTEROBACTERIACEAE ARE REDUCED WHEN HIDES ARE WASHED AND TREATED WITH CETYLPYRIDINIUM CHLORIDE AT A COMMERCIAL BEEF PROCESSING PLANT - (Peer Reviewed Journal)

Bosilevac, J.M., Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Prevalence of Escherichia coli O157 and levels of aerobic bacteria and Enterobacteriaceae are reduced when hides are washed and treated with cetylpyridinium chloride at a commercial beef processing plant. Journal of Food Protection. 67(4):646-650.

PREVALENCE OF ESCHERICHIA COLI O157:H7, LISTERIA MONOCYTOGENES, AND SALMONELLA IN TWO GEOGRAPHICALLY DISTANT COMMERCIAL BEEF PROCESSING PLANTS IN THE UNITED STATES - (Peer Reviewed Journal)

Rivera Betancourt, M., Shackelford, S.D., Arthur, T.M., Westmoreland, K.E., Bellinger, G., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Prevalence of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella in two geographically distant commercial beef processing plants in the United States. Journal of Food Protection. 67:295-302.

PROTOCOL FOR EVALUATING THE EFFICACY OF CETYLPYRIDINIUM CHLORIDE AS A BEEF HIDE INTERVENTION - (Peer Reviewed Journal)

Bosilevac, J.M., Wheeler, T.L., Rivera-Betancourt, M., Nou, X., Arthur, T.M., Shackelford, S.D., Kent, M.P., Jaroni, D., Osborn, M.S., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Protocol for evaluating the efficacy of cetylpyridinium chloride as a beef hide intervention. Journal of Food Protection. 67(2):303-309.

SEASONAL PREVALENCE OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI, INCLUDING O157:H7 AND NON-O157 SEROTYPES, AND SALMONELLA IN COMMERCIAL BEEF PROCESSING PLANTS - (Peer Reviewed Journal)

Gallagher, G.A., Arthur, T.M., Rivera-Betancourt, M., Nou, X., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2003. Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. Journal of Food Protection. 66(11):1978-1986.

DEVELOPMENT OF METHODS FOR THE RECOVERY OF ESCHERICHIA COLI O157:H7 AND SALMONELLA FROM BEEF CARCASS SPONGE SAMPLES AND BOVINE FECAL AND HIDE SAMPLES - (Peer Reviewed Journal)

Barkocy-Gallagher, G.A., Berry, E.D., Rivera Betancourt, M., Arthur, T.M., Nou, X., Koohmaraie, M. 2002. Development of methods for the recovery of Escherichia coli O157:H7 and Salmonella from beef carcass sponge samples and bovine fecal and hide samples. Journal of Food Protection. 65(10):1527-1534