Technology
Analyses and Technical Support
Molecular Mass Determinations of Organic Molecules, Peptides, Protein Digests, and Intact Proteins (API 365 TQMS)
- Electrospray Ionization and Direct Infusion
- Small ionizable molecules
- Semi-purified and pure bio-molecules
- Limited peptide and protein mixtures
- Chromatography-Coupled Electrospray
- Samples with salt and other contaminants
- Complex mixtures and digests not amenable to direct infusion
- Automated High Throughput Mass Analyses by Electrospray
- Samples without detrimental salts by direct flow injection
- Samples with salts removable with reverse phase trapping
- MALDI
- Crude mixtures not amenable to electrospray
- Tryptic digest analysis
Other Techniques (Presently Require Picomolar Amounts)
- Estimation of peptide and protein purity by electrospray
- Disulfide bond analysis
- Chemically modified amino acids
- MS/MS product ion analysis for structural information
Q-TOF2 Hybrid MS for Protein Identification and
Characterization (Shared Instrumentation, Bldg. 8)
- Identification of proteins by peptide map LC/MS and combined LC/MS/MS (amino acid sequence data) from complex sources
- Presently analyses are available for gel extracted protein digests and solution digests at low picomole amounts
- Amenable to extensive peptide mapping of protein digests
- Suited for de novo peptide sequencing
Qstar XL
- Flexible MS/MS platform for both nano-electrospray coupled with LC Packings nano-flow HPLC system and o-MALDI analysis for rapid femtomole sensitivity protein identification
- Suited for de novo peptide sequencing and extensive peptide mapping of protein digests
- High sensitivity and accurate mass accuracy yield unequivocal molecular weights and protein structural information
- Bioanalyst, Pro ID, and Pro ICAT software for advanced and automated analysis of mass spectrometry data
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