ARS | Publication request: Ten microsatellite loci from Zamia integrifolia (Zamiaceae)

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 8, 2007
Publication Date: August 27, 2007
Citation: Meerow, A.W., Nakamura, K. 2007. Ten microsatellite loci from Zamia integrifolia (Zamiaceae). Molecular Ecology Notes. 7(5):824-826.

Interpretive Summary: We isolated 10 DNA markers and designed primers for their amplification from the genome of Zamia integrifolia, the coontie, a popular native ornamental cycad that is considered endangered throughout Florida. This species is part of the poorly understood Z. pumila complex, consisting entireley of populations from the Caribbean and southeastern U.S. The primers have also worked successfully on other species in the complex. We plan to use these markers for populations genetic and characterization studies of the entire Caribbean complex.

Technical Abstract: Ten microsatellite loci isolated from Zamia integrifolia are described. All 10 are polymorphic, with three to ten alleles across 36 members of a single population from South Florida. Heterozygosity ranged from 0.067 to 1. Two loci depart significantly from Hardy Weinberg Equilibrium, and exhibit heterozygote deficiency. One locus pair exhibits significant linkage disequilibrium. The primers have also successfully amplified loci from Z. portoricensis and Z. ambliphyllidia. These loci will be utilized for population studies in the Caribbean Zamia pumila complex.