Docket Number: 11006
Serial Number: 11445091

Technology Description:   ARS researchers have developed a quick and easy extraction method to improve detection of antibodies, antigens, bacteria, and viruses.  The purpose of this technology is to obtain clear aqueous samples in a few steps that can be applied to a variety of diagnostic tests.  For example, this technique affords early, low cost detection of poultry diseases.  Antigens and antibodies are not diluted; therefore, this allows for more sensitive testing.  The advantages of this technology over existing technologies are quick extraction time (less than 30 minutes); clear, clean, concentrated or undiluted extract; high yield of extracted antigen or antibody; ability to use  extract in conventional tests and fluorescence polarization tests; no enrichment step, and inactivation of organisms such as Salmonella.  Existing technologies use harsh chemicals for extraction, purification methods that require numerous steps, and require more than three days for detection of Salmonella from eggs when serotyping is used.               Egg producer, poultry breeder, diagnostic, and vaccine companies could use this technology.  The animal health industry will also benefit from this information.    Reference:   Please refer to patent application S.N. 11/445,091 (Docket #0110.06), “Extraction and Concentration Method,” which was filed on May 31, 2006, and is a continuation of S.N. 10/225,419 (Docket #0221.01), which was filed on August 22, 2002.  Foreign rights are not available.   Inventors:   Henry D. Stone (Collaborator) Egg Safety and Quality Research 950 College Station Road Athens, GA  30605-2796 Phone: (706) 546-3034     Fax: (706) 546-3035 hstone@seprl.usda.gov   Peter S. Holt Egg Safety and Quality Research 950 College Station Road Athens, GA  30605 Phone: (706) 546-3442    Fax: (706) 546-3035 pholt@seprl.usda.gov     Cam R. Greene Exotic and Emerging Avian Viral Diseases 934 College Station Road Athens, GA  30605 Phone: (706) 546-3441    Fax: (706) 546-3161 cgreene@seprl.usda.gov   Richard K. Gast (Same as first inventor) Phone: (706) 546-3445    Fax: (706) 546-3035 rgast@seprl.usda.gov   Kuhno Seo OPDFB, CFSAN, FDA 500 Paint Branch Parkway Room 4E-015, HFS-302 College Park, MD  20740