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Protein Expression
Click for a larger view. (http://www.niehs.nih.gov/research/atniehs/core/pe/images/slide2a.gif) The generation of each expression vector is fast (~2 hrs) and can be done in parallel. Once the Entry clone is made, there are no traditional sub cloning steps in making expression vectors. This removes all problems that may arise due to conflicts with restriction sites naturally occurring within the gene. The gene is always in frame with all tags on all the expression vectors. 
Click for a larger view. (http://www.niehs.nih.gov/research/atniehs/core/pe/images/slide2b.gif) 
Click for a larger view. (http://www.niehs.nih.gov/research/atniehs/core/pe/images/slide2c.gif) |
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