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Abstract
Grant Number: 1F37LM008333-01 Project Title: Identification of Programmed Frameshift Signals in Yeast
PI Information: Name Title JACOBS, JONATHAN L. jacobsjo@wam.umd.edu Abstract: DESCRIPTION (provided by applicant): Proof of principle experiments have shown that -1 programmed ribosomal frame shift signals can act as mRNA suicide elements capable of directing messages to the nonsense mediated mRNA decay pathway. Preliminary data has shown that eukaryotic mRNA on which frame shifted ribosomes encounter premature stop codons are subsequently targeted for rapid degradation. Thus, PRF signals may be utilized as a means to post-transcriptionally regulate gene expression by altering the half-life of cellular mRNAs. The proposed research project utilizes bioinformatics methodologies to identify programmed -1 ribosomal frame shift signals from several eukaryotic model organisms. A bioinformatics protocol for the identification PRF signals is in development. Results will be correlated with a series of DNA microarray experiments designed to detect PRF signal containing mRNA transcripts in Saccharomyces cerevisiae. This will provide validation for the bioinformatics results and a resource for refinement of the PRF signal model. A searchable online database will be created for the scientific community to utilize. The proposed studies will serve as the foundation for further research into a newly discovered paradigm of gene regulation.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
computer assisted sequence analysis, fungal genetics, genetic regulatory element, genetic transcription, messenger RNA, ribosome
Internet, bioinformatics, computational biology, computer program /software, computer system design /evaluation, eukaryote, molecular biology information system, nucleic acid sequence
Saccharomyces cerevisiae, gene expression profiling, microarray technology
Institution: UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS 3112 LEE BUILDING COLLEGE PARK, MD 207425141 Fiscal Year: 2004 Department: CELL BIOLOGY AND MOLECULAR GENETICS Project Start: 15-APR-2004 Project End: 14-APR-2007 ICD: NATIONAL LIBRARY OF MEDICINE IRG: BLR