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DOE Human Genome Program Contractor-Grantee Workshop IV

Santa Fe, New Mexico, November 13-17, 1994

Introduction to the Workshop
URLs Provided by Attendees

Abstracts
Mapping
Informatics
Sequencing
Instrumentation
Ethical, Legal, and Social Issues
Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Sequencing

Department of Energy Human Genome Program
Contractor-Grantee Workshop IV
Santa Fe, NM; November 13-17, 1994

Preparation of Oligonucleotide Arrays for Hybridization Studies
Michael C. Pirrung, Steven W. Shuey, David C. Lever, Lara Fallon, J.-C. Bradley, and William P. Hawe

Oligonucleotide Microchip is a Versatile Tool for DNA Analysis: Diagnostic Applications
Igor Ivanov, Eugene Kirillov, Victor E.Barsky, Edward Kreindlin, Sergei Parinov, Edward Timofeev, Gennady Yershov, Vladimir L. Florentiev, and Andrei D. Mirzabekov

Electronically controlled DNA hybridization on semiconductor microchips: A miniaturized DNA chip format for rapid medical diagnostics and DNA sequencing
Glen A. Evans, Harold R. Garner, Eugene Tu, William F. Butler, Ronald G. Sosnowski, Donald D. Montgomery and Michael J. Heller

Positional Effects of Nearest-Neighbor Base Pairs and Mismatched Base Pairs In Short DNA Duplexes
Mitchel J. Doktycz, Maxwell Morris, K. Bruce Jacobson, Kenneth L. Beattie, and Robert S. Foote

Accessing Genetic Information with DNA Arrays
Stephen P.A. Fodor and Robert Lipshutz

Sequencing by Hybridization: Towards an Efficient Large-Scale DNA Methodology
Radomir Crkvenjakov

Development of Instrumentation for DNA Sequencing at a Rate of 40 Million Bases per Day
Edward S. Yeung, Huan-Tsung Chang, Qingbo Li, Xiandan Lu

Capillary Array Electrophoresis for High Throughput DNA Analysis
John Bashkin, David Barker, Dave Roach, Matt Bartosiewicz, Joe Leong, Tom Zarella, Rick Johnston

Multiple Capillary DNA Sequencing
Norman J. Dovichi, Jian-Zhong Zhang, Jian-Ying Zhao, Jiang Rong, Rong Liu, John Elliott, Sue Bay, Pieter Roos, and Larry Coulson

DNA Analysis by Capillary Electrophoresis: Sequencing and Mutant Detection
Barry L. Karger, Jan Berka, Marie C. Ruiz-Martinez, Steve Carson, Appasani S.M. Krishnarao, Kirstin Hebenbrock and Frantisek Foret

New Directions in High-Sensitivity Fluorescence Detection of DNA and Capillary Array Electrophoresis
Richard A. Mathies, Jingyue Ju, Huiping Zhu, Steven M. Clark, Adarn T. Woolley, Yiwen Wang, Scott C. Benson and Alexander N. Glazer

Sequencing of DNA by Gel Electrophoresis in Micromachined Channels
Joe Balch, Courtney Davidson, Jeff Gingrich, Muhammad Sharaf, Larry Brewer, Jackson Koo, Doug Smith, Michael Albin, and Anthony Carrano

High-Speed Automated DNA Sequencer Utilizing From-the-Side Laser Excitation
Michael S. Westphall, Robert L. Brumley Jr., Eric C. Buxton, and Lloyd M. Smith

Fluorescent Sequencer Development
William F. Kolbe and Jocelyn C. Schultz

Design, Synthesis and Characterization of Energy Transfer Fluorescent Dye Tagged Oligonucleotide Primers for DNA Sequence Analysis
Jingyue Ju, Chihchuan Ruan, Carl W. Fuller, Alexander N. Glazer and Richard A. Mathies

Luminescent Lanthanide Ions as Labels for DNA Sequencing and Mapping
Gilbert M. Brown, Jeffrey E. Elbert, Frederick V. Sloop, Mitchel J. Doktycz, Richard A. Sachleben, and K. Bruce Jacobson

Laser Desorption Mass Spectrometry for Fast DNA Sequencing
C. H. Winston Chen, Kai Tang, Nelli I. Taranenko, and Steve L. Allman

Progress in DNA sequence mixture readout by mass spectrometry
Chau-Wen Chou, David Dogruel, Jennifer Krone, Randall Nelson, David Schieltz and Peter Williams

Fragmentation Studies of Oligonucleotides Using Matrix-Assisted Laser Desorption Mass Spectrometry
Christine M. Nelson, Lin Zhu, Stephane Mouradian, Wei Tang and Lloyd M. Smith

Analysis of Oligonucleotide Mixtures by Electrospray Ionization-Mass Spectrometry
Xueheng Cheng, David C. Gale, Harold R. Udseth and Richard D. Smith

High Speed Sequencing of Single DNA Molecules in the Gas Phase by FTICR-MS
Richard D. Smith, Xueheng Cheng, S. A. Hofstadler, J. A. Bruce and Charles G. Edmonds

Electrophore-Labeled DNA for Enhanced Sensitivity in Matrix-Assisted Laser Desorption Mass Spectrometry
Phillip F. Britt, Gregory B. Hurst, and Michelle V. Buchanan

Human DNA Sequencing at the LBL Human Genome Center
Michael Palazzolo, Christopher Martin, William Kimmerly, Edward Rubin, J. F. Cheng, Joseph Jaklevic, Edward Theil, and Mohan Narla

Directed Genomic DNA Sequencing
Christopher H. Martin, Cheryl A. Davis, Carol A. Mayeda, Herb Moise and Michael J. Palazzolo

Large-scale Sequencing of the Human and Mouse T Cell Receptor Loci
Leroy Hood, Lee Rowen, Kai Wang, Inyoul Lee, Cecilie Boysen, and Ben F. Koop

Automated Fluorescent Detection for Multiplex DNA Sequencing
Robert B. Weiss, F. Mark Ferguson, Leonard Di Sera, Alvin Kimball, Josh Cherry, Mark Stump, Andy Marks, Tony Schurtz, Diane Dunn and Raymond F. Gesteland

The C. elegans Genome Sequencing Project.
Richard K. Wilson and the C. elegans Genome Consortium.

Whole Genome Shotgun Sequencing of the 2.0 Mb Genome of Haemophilus influenzae
Carol J. Bult, Robert D. Fleischmann, Mark D. Adams, Joseph M. Merrick, Jeannine Gocayne, Li-ing Liu, Anthony Kerlavage, Hamilton O. Smith, and J. Craig Venter

Microbial Genome Sequencing
Douglas R. Smith and John Reeve

High-Throughput DNA Sequencing and Characterization of Diverse Microbial Genomes
J. Craig Venter and Carl Woese

Automated Multiplex Microbial Genome Sequencing
Joshua L. Cherry, Debi Nelson, Peter Cartwright, Mark Stump, Diane Dunn and Robert B. Weiss

Genomic Sequencing of Human and Rodent DNA Repair Gene Regions
Jane E. Lamerdin, Mishelle A. Montgomery, Stephanie A. Stilwagen, Jakob M. Kirchner, Edmund P. Salazar, Christine A. Weber, Robert S. Tebbs, Kerry W. Brookman, Larry H. Thompson, and Anthony V. Carrano

Evaluation of High Throughput M13 DNA Isolation Protocols
Maria de Jesus, Alex Copeland, Jane Lamerdin, Anthony V. Carrano, and Ray Mariella

Progress Toward Automation of the Front-End of DNA Sequencing
Richard A. Guilfoyle, Jim Uzgiris, Huamin Ji, Qinghua Liu, Dan Chen, Michael Westphall, Robert Brumley, Brian Boville, Jessica Hayden, Zhen Guo, Andy Thiel, Arthur Johnson, Todd Francisco, Ricardo Gee, and Lloyd M. Smith

Studies of T7 DNA Polymerase to Improve the Properties of DNA Sequencing Enzymes
Stanley Tabor, Jeff Himawan, and Charles C. Richardson

Design of a Parallel Array Oligonucleotide Synthesizer
Thomas Brennan, Scott Hunicke-Smith, Deval Lashkari, and Ronald Davis

Using Expressed Sequences as Nucleation Points for Genomic Sequencing
Michael R. Altherr, Amanda Ford, Cleo Naranjo, Judy Buckingham, Chris Munk and Robert K. Moyzis

Application of Single Molecule Detection to DNA Sequencing and Sizing
W. Patrick Ambrose, Peter M. Goodwin, James H. Jett, Mitchell E. Johnson, John C. Martin, Babetta L. Marrone, Jeffery T. Petty, Jay A. Schecker, Ming Wu, Richard A. Keller, Alberto Haces, Po-Jen Shih, and John D. Harding

Toward an Automated System for High-Throughput DNA Sequencing: 1. Primer Walking from a Hexamer Library
Jan Kieleczawa, Shiping Zhang, John J. Dunn and F. William Studier

Toward an Automated System for High-Throughput DNA Sequencing: 2. Multiple Capillary Electrophoresis with a Replaceable Matrix
Mark A. Quesada, Jan Kieleczawa, Shiping Zhang, John J. Dunn and F. William Studier

Toward an Automated System for High-Throughput DNA Sequencing: 3. Fesmid Vectors for Sequencing by Primer Walking
John J. Dunn and F. William Studier

Modular Primers in Automated DNA Sequencing
Levy Ulanovsky, Irina Sobolev & Lev Kotler

One-Step PCR Sequencing
Ken Porter, David Briley, and Barbara Ramsay Shaw

Quantitative Comparison of the Incorporation of Borano- and Thio- Deoxynucleoside Triphosphate Analogs by Klenow Polymerase
Kenneth Porter, Katie Ealey, J. David Briley, Faqing Huang and Barbara Ramsay Shaw

Improvement of Ligation-Mediated PCR for DNA Sequence Determination
Kenneth S. Graham, Arian F.A. Smit, and Arthur D. Riggs

Fingerprinting, clustering and sequence recognition by hybridization to short oligomers: informational support for targeted cDNA and genomic sequencing
A. Milosavljevic, S. Savkovic, S. Batus, H. Dyanov, A. Gemmell, D. Grujic, D. Salbego, M. Zeremski, Z. Strezoska, T. Paunesku, S. Little, H. Kreuzer, and H. Serrato, R. Crkvenjakov

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