Imrie A, Hurren L, Cunningham P, Vizzard J, Carr A, Gurusinghe A, Deacon N, Penny R, Cooper DA; Australasian Society for HIV Medicine. Conference.
Annu Conf Australas Soc HIV Med. 1993 Oct 28-30; 5: 36 (abstract no. SC4).
Centre for Immunology, St Vincents's Hospital.
AIM: To assess the prevalence of transmitted zidovudine-resistant HIV in individuals who seroconverted after 1990. METHODS: HIV was isolated from 10 homosexual men with symptomatic primary HIV infection, and from their sexual partners when possible. Isolates obtained within the first two months following onset of symptoms were assessed for ZDV sensitivity with a PBL assay. HIV RT from one donor-recipient pair was sequenced. RESULTS: Isolates obtained within a mean of 16.5 (r8-32) days of onset of symptoms demonstrated a mean ZDV IC50 of 0.008 (r0.001-0.041); IC50 of isolates from 3 sexual partners ranged from 0.001 to 0.08. Sequence analysis of the HIV RT of one donor-recipient pair with IC50 values of 0.08 and 0.01 respectively showed a mutation at position 70 (Lys-Arg) in the donor RT; the RT of his sexual partner showed a wild-type sequence. CONCLUSION: HIV with reduced sensitivity to ZDV was isolated from a homosexual man, but not from his seroconverting sexual partner. Phenotypic and genotypic analysis of additional PHI isolates and donor-recipient pairs will be presented.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Male
- Sexual Partners
- Zidovudine
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102207948
From Meeting Abstracts