Gounder C, Carvalho de Queiroz Mello F, Kritski A, Chaisson R; International Conference on AIDS.
Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. MoPeB2308.
C. Gounder, Johns Hopkins School of Hygiene and Public Health, 67 W 73rd St., Apt. 2D, New York, NY 10023, United States, Tel.: +1 212 579 4785, E-mail: gounder@alumni.princeton.edu
Background: To correlate the results of a rapid immunochromatographic test (IT) for tuberculosis (TB) in whole blood, plasma and serum with TB culture results. Keywords: tuberculosis, diagnostics, serology Methods: The IT detects IgG responses to 4 recombinant M. tuberculosis complex proteins. Participants were drawn from the Hospital Universitario Clementino Fraga Filho in Rio de Janeiro, and the Centro Municipal de Saude Belizario Penna da XVIII RA in Campo Grande, Brazil. Subjects included 70 patients with a suspicion of pulmonary TB and for whom TB cultures were ordered, 21 healthy subjects with no BCG scar who were PPD- (Group A), and 21 healthy subjects with a BCG scar and/or who were PPD+ (> = 10 mm) (Group B). Serum, whole blood and plasma from each subject were run on the IT as soon as possible on the day of collection. TB culture was the reference standard for TB suspects. Results: For Group A, the specificity of the IT in whole blood, serum and plasma respectively was: 95.2%, 100.0% and 95.2%. For Group B, the specificity of the IT in these same specimen types respectively was: 71.4%, 85.7% and 85.7%. Among TB suspects, the sensitivity of the IT in whole blood, serum and plasma respectively was: 83.3%, 65.0% and 70.0%, and the specificity was: 45.9%, 67.5% and 53.7%. Among AFB smear+ TB suspects, the sensitivity of the IT in whole blood, serum and plasma respectively was: 100.0%, 78.6% and 85.7%. Among the 16 HIV+ TB suspects, the sensitivity of the IT in whole blood, serum and plasma respectively was: 80.0%, 40.0% and 40.0%. Conclusions: The IT is promising because it requires no laboratory infrastructure, can be run in 15 minutes allowing for diagnosis in a single clinic visit, and can be done by staff with minimal training. Specificity of the IT must be improved in certain subpopulations. The IT should be further evaluated as a potential substitute for AFB smears, and as a potential criterion for patient isolation.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- BCG Vaccine
- Bacteriological Techniques
- Brazil
- Evaluation Studies
- HIV Infections
- Humans
- Patient Isolation
- Sensitivity and Specificity
- Serologic Tests
- Tuberculin
- Tuberculin Test
- Tuberculosis
- Tuberculosis, Pulmonary
- immunology
- methods
Other ID:
UI: 102237908
From Meeting Abstracts