Parravicini C, Kaaya E, Gendelman R, Berti E, Nakamura S, Ensoli B, Gallo RC, Biberfeld P; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 117 (abstract no. W.A.1103).
V Dep. of Pathology, State University, Ospedale L.Sacco, Milano, Italy
OBJECTIVES AND METHODS: To characterize by immunocyto/histochemistry KS spindle cells in lesions in comparison to established KS-derived cell culture; to determine the possible expression of HIV antigens by these cells and to determine their ploidy. RESULTS: In vivo, the morphologically defined KS spindle cell compartment was predominantly composed of a mixture of proliferating CD45+/CD68+/CD4+ monocytic cells and TE7+/Collagen+ fibroblastic cells which also could express CD34/EN4 endothelium-associated antigens and/or smooth muscle (SM) specific alpha actin. KS-derived cells grown in vitro were also TE7+, partly SM actin+ like myofibroblasts, but negative for endothelium restricted antigens. By immunohistochemistry of biopsies and cell cultures gag and env HIV-antigens were not demonstrable. However, with antibodies to HIV-1-regulatory gene products variable results were observed in lesions depending on the antibody used. By DNA quantification on both epidemic and endemic KS spindle cells in sections and in culture, the cells were shown to be diploid. CONCLUSION: Our observations indicate that the supposedly tumor, spindle cell compartment of Kaposi's lesions is a heterogeneous mixture of proliferating monocytic and fibroblastic cells with diploid DNA content, suggesting a reactive rather than a clonal (tumor) cell proliferation.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Actins
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- CD68 antigen, human
- HIV Antigens
- HIV Seropositivity
- Immunohistochemistry
- In Vitro
- Killer Cells, Natural
- Macrophages
- Monocytes
- Sarcoma, Kaposi
- immunology
Other ID:
UI: 102192295
From Meeting Abstracts