Guarner J, Unger ER; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 232 (abstract no. M.B.P.62).
Emory University, Atlanta, Georgia, USA
OBJECTIVE: Epithelioid hemangioma-like vascular proliferations, recently described in AIDS patients, have been associated with bacilli similar to those found in cat-scratch disease. Since other vascular lesions present in AIDS patients, in particular Kaposi's sarcoma, have been associated with CMV, we investigated the possibility of viral associations with epithelioid angiomatosis. METHOD: In-situ hybridization was performed on formalin fixed, paraffin embedded tissue from 2 cases using cloned biotinylated probes for CMV, herpes simplex (HSV), HIV and EBV. RESULTS: None of the 2 patients had evidence of opportunistic infection at the time the biopsy was obtained and the lesions regressed spontaneously. Hybridizations with CMV, HSV and HIV were negative, those for EBV were positive in both lesions. Hybridization signal for EBV was present in the nuclei of endothelial cells and in occasional histocytes. Bacilli were demonstrated within only one of the lesions by silver stain. CONCLUSIONS: This is the first report of EBV associated with this entity and the first time that EBV viral genome has been seen in endothelial cells. Our data suggest that EBV may be involved in the pathogenesis of these lesions but further studies are necessary.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Angiomatosis, Bacillary
- Animals
- Cat-Scratch Disease
- Cats
- Genome, Viral
- HIV Seropositivity
- Herpesvirus 4, Human
- Humans
- In Situ Hybridization
- Nucleic Acid Hybridization
- Opportunistic Infections
- Sarcoma, Kaposi
- genetics
Other ID:
UI: 102176915
From Meeting Abstracts