KANSAL R, PARSONNET J, DAVIS C, SEYMOUR J, HANSMANN M, KOTB MY; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. B-1436.
University of Tennessee, Memphis, TN.
BACKGROUND: Superantigens produced by Staphylococcus aureus are believed to play a central role in the pathogenesis of menstrual toxic shock syndrome (mTSS). In particular, the superantigen TSST-1 is associated with virtually all mTSS cases. The presence of antibodies to TSST-1 that can neutralize its superantigenic activity is likely to influence susceptibility to development of mTSS. The possibility that different isotypes of anti-TSST-1 antibodies (Abs) may be closely associated with effective neutralization of TSST-1 activity was investigated. METHODS: Venous blood was collected from 10 women who regularly use tampons at two different time points in their menstrual cycle (days 2-4 and days 12-16). The levels and isotypes of the Abs were determined by ELISA. The functional properties of anti-TSST-1 Abs were determined in a tissue culture neutralization assay. In this assay, the ability of subject plasma to specifically block the proliferative response of healthy donor T cells to TSST-1 without affecting the phytohemaglutinin (PHA) response was evaluated. RESULTS: Data were analyzed with JMP(R)/SAS by statistical modeling of linear regression and curve fitting via least squares methodology. IgG1 and IgG4 were highly correlated with the percent neutralization of TSST-1 (r[2]=0.87, p<0.0001). CONCLUSIONS: A lack of IgG1 and IgG4 isotypes has been associated with the development of mTSS (Clin. Exp. Immunol., 66:443-449, 1986). The results in this study demonstrate that these two isotypes are highly associated with neutralization of TSST-1. Future studies will evaluate this relationship in acute and convalescent phase sera from patients who have been diagnosed with mTSS.
Publication Types:
Keywords:
- Activities of Daily Living
- Antibodies
- Bacterial Toxins
- Enterotoxins
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunoglobulin G
- Shock, Septic
- Staphylococcus aureus
- Superantigens
- T-Lymphocytes
- Tampons, Surgical
- enterotoxin F, Staphylococcal
- immunology
- rehabilitation
Other ID:
UI: 102268016
From Meeting Abstracts