Kopp J, Wohlenberg C, Marinos N, Klotman P, Notkins A, Rooney J; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 191 (abstract no. PO-A17-0342).
NIDR, NIH, Bethesda, MD.
Patients infected with HIV experience an increased prevalence of papulosquamous skin disorders, including seborrheic dermatitis and psoriasis. We have developed a mouse transgenic for a gag-pol deleted HIV-1 proviral construct which expresses viral mRNA and envelope glycoproteins in epidermal cells and develops spontaneously occurring cutaneous papillomas. In the present report, we have identified a variety of wounding and inflammatory stimuli which can induce papilloma formation in a site-specific manner within 14-21 days following application of the stimulus. These stimuli include ultraviolet B light, phenol, liquid nitrogen, dithranol, cutaneous viral infection (HSV and VV) and mild abrasion. Development of papillomas was associated with increased HIV transgene mRNA and envelope protein expression. Other stimuli, such as trinitrochlorobenzene, surgical incision, subcutaneous saline injection, topical steroids, retinoic acid, butyl nitrate, and phorbol ester, did not induce lesion formation, and papillomas did not occur in control nontransgenic littermates treated with any stimulus. This small animal model may prove useful in evaluating the role of HIV gene products in disease pathogenesis and could be used to evaluate therapeutic interventions directed against HIV envelope or regulatory proteins.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Animals, Genetically Modified
- Genes, gag
- Genes, pol
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Mice
- Models, Biological
- Papilloma
- Prevalence
- Skin
- Skin Diseases
- Skin Neoplasms
- Transgenes
- genetics
Other ID:
UI: 102203156
From Meeting Abstracts