Carriere D, Fontaine C, Rouquette AM, Juillard R, Pau B; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 259 (abstract no. M.B.2310).
Sanofi Recherche, Montpellier, France
OBJECTIVE AND METHODS: A simple and sensitive ELISA immunoassay, enabling the determination of CD4 or CD8 antigens on the cell surface of lymphocytes, has been standardized. The assay is performed on microtitration plates in a single step including: 1) immobilization of cells by adsorbed pan-T monoclonal antibodies 2) simultaneous labelling of CD4 or CD8 by peroxydase-labelled immuno-conjugates. RESULTS: They are expressed in molar concentrations of CD4 or CD8 molecules calculated from standard curves. The mean values of CD4 (extreme values in parenthesis) from blood samples from HIV- subjects (n=134), HIV+ subjects (n=97) and AIDS patients (n=62) are 17.5 pH (8-38), 9 pH (2-27) and 3.5 pH (0, 4-8), respectively. The concentrations of CD8 (pH) are not significantly different in the 3 groups. CONCLUSIONS: The proposed CD4/CD8 immunoassay is a fast and sensitive technique easy to perform in routine conditions for the investigation of T-cell populations. It is a very useful tool for the follow-up of HIV infection in AIDS patients.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- Antigens, CD8
- HIV Infections
- HIV Seropositivity
- Humans
- T-Lymphocytes
- immunology
Other ID:
UI: 102183230
From Meeting Abstracts