Tomohiko T, Sano K, Tanaka E, Morimatsu S, Tsurutani R, Kifune K, Nakai M, Yamaguchi J; International Conference on AIDS.

Int Conf AIDS. 1992 Jul 19-24; 8: 39 (abstract no. PuA 6171).

Dept. of Bacteriology, Kinki University School of Medicine, Japan.

OBJECTIVES: We designed an effective virus concentration method to prevent the infection with human immunodeficiency virus (HIV) in laboratories. METHODS: The absorbent of MINICON concentrators (Amicon Division, W.R. Grace & Co. -Conn.) was changed to chitin (chitin sheets: Beschitin, UNITIKA Ltd.), a mucopolysaccharide extracted from the shells of Japanese pink crab. HIV-1 in the supernatant of HIV-1(LAV-1) infected Molt-4 cells were concentrated by Minicon and the new concentrator. The volume of concentrated HIV-1 was checked by p24 antigen capture (ELISA, Abbott). RESULTS: The time to be 1/5 volume of the supernatant were 61.8 +/- 3.0 min. (CV: 0.88) on MINICON, 74.8 +/- 4.6 min. (CV: 0.06) on the new concentrator. And the concentration rate were 3.43 +/- 0.45 times (CV: 0.45) on MINICON, 4.10 +/- 0.34 times (CV: 0.13) on the new concentrator. CONCLUSIONS: The new concentrator using chitin sheets showed good concentration rate and equality of concentration speed. This should be useful to concentrate HIV and detect isolated HIV.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Chitin
• Enzyme-Linked Immunosorbent Assay
• HIV
• HIV Antibodies
• HIV Antigens
• HIV Core Protein p24
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• immunology
• methods

Other ID:

• 92404250

UI: 102201964

From Meeting Abstracts