MELETIADIS J, CURFS J, MEIS JF, VERWEIJ PE; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 384.
Univ. Med. Ctr. Nijmegen, Nijmegen, The Netherlands
BACKGROUND: Scedosporium prolificans may cause invasive infections in immunocompromised individuals. This pathogen is highly refractory to antifungal drugs and very few experimental data exist about the pathogenicity of this fungus. In this study a murine model for invasive scedosporiosis was developed in order to evaluate different treatment regimens.METHODS: 6-8 weeks old CD-1, CBA, Balb/c and C57 female mice weighing approximately 20gr were infected with different inocula ranging from 10[2] to 10[8 ]CFU per mouse. Both neutropenic and non-neutropenic mice were used. For the immunosuppression, cyclophosphamide was administered subcutaneously at a dose of 200mg/kg. The mice were infected by 4 different routes: intraperitoneally (ip), intramuscularly (im) and intravenously by the orbital vein (iv-o) and the tail vein (iv-t). The animals were observed daily for 15 days. Survival rates were record and the following organs were processed for histology and cultures: kidney, spleen, lung, liver and brain.RESULTS: CD-1, Balb/c and C57 mice were resistant to infections caused by 10[4] CFU per mouse compared with the CBA mice which died on days 4-6 after the inoculation. The iv injection was fatal and no mortality was observed when other routes of inoculation were used. The most infected organs were the brain (54+/- 17x10[3 ]CFU/gr) followed by the liver (5,6+/- 1,1x10[3 ]CFU/gr). The level of immunosuppression appeared to have no effect on the fatality of the infection.CONCLUSIONS: A murine model using CBA mice was developed for invasive scedosporiosis, both in neutropenic and non-neutropenic mice. This model can be used for the investigation of efficacy of management regimens for infections caused by S. prolificans.KEYWORDS: Murine model; Scedosporiosis
Publication Types:
Keywords:
- Animals
- Antifungal Agents
- Cyclophosphamide
- Disease Models, Animal
- Female
- Humans
- Immunocompromised Host
- Immunosuppression
- Infection
- Kidney
- Maduromycosis
- Mice
- Mice, Inbred CBA
- Models, Animal
- Muridae
- Opportunistic Infections
- Scedosporium
- etiology
- immunology
Other ID:
UI: 102247793
From Meeting Abstracts