ANDRADE R, CULEBRAS E, BETRIU C, ARANDA G, REDONDO M, PEREZ-CECILIA E, PICAZO JJ; ACINETOBACTER GROUP; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. C2-2228.
Hospital Clinico San Carlos, Madrid, Spain
BACKGROUND: The multiresistant strains of Acinetobacter spp. are begining to be a serious problem in our country. The aim of this study was to know the in-vitro antimicrobial susceptibility of carbapenem-resistant A. baumannii strains and to detect the presence of metallo-beta-lactamase gene bla[IMP]. METHODS: A total of 61 carbapenem-resistant isolates were studied. All of them were collected from different patients at 13 Spanish hospitals. The following antibiotics were used: cefotaxime (CTX), ceftazidime (CAZ), gentamicin (GEN), amikacin (AMK), ciprofloxacin (CIP), levofloxacin (LEV), ampicillin (A), ampicillin/sulbactam (A/S), piperacillin/tazobactam (P/T), doxycycline (DOX), colistin (COL), rifampicin (RIF), and cotrimoxazol (COT). MICs were determined by the agar dilution method; bla[IMP] gene was detected by PCR using specific primers. RESULTS: the susceptibility patterns were as follows: [table: see text] 96.7% of strains were inhibited by colistin at = 1microg/ml. The bla[IMP ]gene was detected in 77% strains. CONCLUSIONS: 1. Carbapenem-resistant A.baumannii isolates are resistant to multiple antibiotics. 2. Colistin showed the best activity of all antimicrobials tested, followed by doxicyclyne and ampicillin/sulbactam. 3. The metallo beta-lactamase (IMP-1) encoded by bla[IMP ]gene could have an important role in the resistance to carbapenems and broad-spectrum beta-lactams in these microorganisms. 4. To our knowledge, this is the first detection of bla[IMP ]gene in Spanish A.baumannii isolates.>
Publication Types:
Keywords:
- Acinetobacter
- Acinetobacter Infections
- Acinetobacter baumannii
- Ampicillin
- Anti-Bacterial Agents
- Carbapenems
- Ceftazidime
- Colistin
- Humans
- Microbial Sensitivity Tests
- Sulbactam
- beta-Lactamases
- beta-Lactams
- sultamicillin
Other ID:
UI: 102268913
From Meeting Abstracts