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IMAGEJ archives -- July 2004 (#60)

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Date:         Fri, 9 Jul 2004 17:52:20 -0400
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Sender:       ImageJ Interest Group <[log in to unmask]>
From:         "Herbert M. Geller" <[log in to unmask]>
Subject:      Re: SDS-PAGE: Molecular Weight Calibration
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Wayne,
    I think the problem that is posed is to estimate the molecular
weight of a protein sample based on the position of the band in a gel
relative to molecular weight markers.
    This would probably require some non-linear curve fitting of the
distance along the gel vs. the MW of the markers.  Then the distance to
the unkown band can be plugged in to get the estimated MW.
Herb Geller

Wayne Rasband wrote:

>> I am new to ImageJ.
>> I would like to measure the sizes of protein bands in a gel.
>> Which micro do I need?
>
>
> You can measure the relative size of protein bands with the aid of
> commands in the Analyze>Gels sub-menu. The procedure is described at:
>
>      http://rsb.info.nih.gov/ij/docs/menus/analyze.html#gels
>
> -wayne


--
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Herbert M. Geller, Ph.D.
Associate Director for Education
Division of Intramural Research
National Heart Lung and Blood Institute
National Institutes of Health
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Bethesda, MD  20892-1754
Tel: 301-451-9440
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